Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using MBS7602297 (P16-INK4A Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9) .)
Rabbit P16-INK4A Polyclonal Antibody | anti-P16-INK4A antibody
P16-INK4A Rabbit Polyclonal
Reactivity
Human, Mouse, Rat; other species are not tested.
Applications
ELISA, Western Blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
>=95% as determined by SDS-PAGE; Immunogen affinity purified
Synonyms
P16-INK4A; Polyclonal Antibody; P16-INK4A Rabbit Polyclonal; ARF; CDKN2A; CDK4 inhibitor p16 INK4; CDK4 inhibitor p16INK4; CDK4I; CDKN2; CMM2; INK4; INK4a; MLM; MTS 1; MTS1; Multiple tumor suppressor 1; p14; p14ARF; P16; p16 INK4; p16 INK4a; p16INK4; p16INK4a; P19; TP16; anti-P16-INK4A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat; other species are not tested.
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, Mouse, Rat; other species are not tested. Please decide the specificity by homology
Purity/Purification
>=95% as determined by SDS-PAGE; Immunogen affinity purified
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Applicable Applications for anti-P16-INK4A antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:50-1:500
IF: 1:20-1:200
IP: 1:500-1:5000
IHC: 1:50-1:500
IF: 1:20-1:200
Purification
Immunogen affinity purified
Purity
> 95% as determined by SDS-PAGE
Immunogen
Cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
Preparation and Storage
-20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using MBS7602297 (P16-INK4A Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9) .)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using MBS7602297 (P16-INK4A Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9) .)
Immunoprecipitation (IP)
(IP Result of anti-P16 (IP: MBS7602297, 4ug; Detection: MBS7602297 1:1000) with HEK-293 cells lysate 4500ug.)
Immunoprecipitation (IP)
(IP Result of anti-P16 (IP: MBS7602297, 4ug; Detection: MBS7602297 1:1000) with HEK-293 cells lysate 4500ug.)
Western Blot (WB)
(HEK-293 cells were subjected to SDS PAGE followed by western blot with MBS7602297 (P16 antibody) at dilution of 1:1000)
Western Blot (WB)
(HEK-293 cells were subjected to SDS PAGE followed by western blot with MBS7602297 (P16 antibody) at dilution of 1:1000)
Related Product Information for anti-P16-INK4A antibody
CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively-spliced variants encoding distinct proteins have been reported, and two are related,
named p16-INK4 and p14 respetively, sharing 50% identity. The third one is completely stuctually un-related, called p14(ARF) or p19(ARF). FNab06064 reacted with p16 specifically. P16 plays an important role in regulating the cell cycle, and mutations in p16 increase the risk of developing a variety of cancers, notably melanoma.
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The P16-INK4A (Catalog #AAA7602297) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The P16-INK4A Rabbit Polyclonal reacts with Human, Mouse, Rat; other species are not tested. and may cross-react with other species as described in the data sheet. AAA Biotech's P16-INK4A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:5000 IP: 1:500-1:5000 IHC: 1:50-1:500 IF: 1:20-1:200. Researchers should empirically determine the suitability of the P16-INK4A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P16-INK4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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