Western Blot (WB)
(Anti-EIF5A2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: CaCo 2 Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:19 kDa)
Rabbit anti-Human EIF5A2 Polyclonal Antibody | anti-EIF5A2 antibody
Anti-EIF5A2 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 0.5-1uL/mg of lysate
Western Blot (WB)
(Anti-EIF5A2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: CaCo 2 Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:19 kDa)
Western Blot (WB)
(Anti-EIF5A2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: CaCo 2 Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:19 kDa)
Immunoprecipitation (IP)
(EIF5A2 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-EIF5A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-EIF5A2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa Observed band size :17 kDa)
Immunoprecipitation (IP)
(EIF5A2 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-EIF5A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-EIF5A2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa Observed band size :17 kDa)
NCBI and Uniprot Product Information
Uniprot Description
EIF5A2: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Belongs to the eIF-5A family.
Chromosomal Location of Human Ortholog: 3q26.2
Cellular Component: endoplasmic reticulum membrane; nuclear pore; cytosol
Molecular Function: protein binding; ribosome binding; translation elongation factor activity
Biological Process: protein transport; mRNA transport; translational frameshifting; cellular protein metabolic process; positive regulation of cell proliferation; positive regulation of translational elongation; spermatogenesis; hypusine biosynthetic process from peptidyl-lysine; polyamine homeostasis; post-translational protein modification; positive regulation of translational termination
Research Articles on EIF5A2
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Product Notes
The EIF5A2 eif5a2 (Catalog #AAA8107502) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EIF5A2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF5A2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 0.5-1uL/mg of lysate. Researchers should empirically determine the suitability of the EIF5A2 eif5a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF5A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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