Rabbit anti-Human PHGDH Polyclonal Antibody | anti-PHGDH antibody

Anti-PHGDH Antibody, Rabbit Polyclonal

Cat. Num. AAA8106793

• Gene Names: PHGDH; NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PHGDH; Polyclonal Antibody; Anti-PHGDH Antibody; Rabbit Polyclonal; PGDH/PHGDH Antibody; Rabbit PAb; Antigen Affinity Purified; phosphoglycerate dehydrogenase; Anti-3-PGDH Antibody; Anti-3PGDH Antibody; Anti-HEL-S-113 Antibody; Anti-NLS Antibody; Anti-NLS1 Antibody; Anti-PDG Antibody; Anti-PGAD Antibody; Anti-PGD Antibody; Anti-PGDH Antibody; Anti-PHGDHD Antibody; Anti-SERA Antibody; anti-PHGDH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PGDH/PHGDH
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PHGDH antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 4-8uL/mg of lysate
• Immunogen: Recombinant Human PGDH/PHGDH Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human PGDH/PHGDH (rh PGDH/PHGDH). PGDH/PHGDH specific IgG was purified by Human PGDH/PHGDH affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(PHGDH was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg A431 Whole Cell Lysate4 uL anti-PHGDH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-PHGDH rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size: 58 kDa)

Western Blot (WB)

(Anti-PHGDH rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: A431 Whole Cell LysateLane C: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:57 kDaObserved band size:57 kDa)

Related Product Information for anti-PHGDH antibody

PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+ /NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.

References

Pind S, et al. (2002) V490M, a common mutation in 3-phosphoglycerate dehydrogenase deficiency, causes enzyme deficiency by decreasing the yield of mature enzyme. J Biol Chem. 277 (9): 7136-43.Du H, et al. (2010) 3-Phosphoglycerate dehydrogenase expression is regulated by HOXA10 in murine endometrium and human endometrial cells. Reproduction. 139 (1): 237-45.Possemato R, et al. (2011) Functional genomics reveal that the serine synthesis pathway is essential in breast cancer. Nature. 476 (7360): 346-50.

NCBI and Uniprot Product Information

• NCBI GI #: 21264510
• NCBI GeneID: 26227
• UniProt Accession #: O43175; Q5SZU3; Q9BQ01; B2RD08
• Molecular Weight: 533
• NCBI Official Full Name: D-3-phosphoglycerate dehydrogenase
• NCBI Official Synonym Full Names: phosphoglycerate dehydrogenase
• NCBI Official Symbol: PHGDH
• NCBI Official Synonym Symbols: NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
• NCBI Protein Information: D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113
• UniProt Protein Name: D-3-phosphoglycerate dehydrogenase
• UniProt Gene Name: PHGDH
• UniProt Synonym Gene Names: PGDH3; 3-PGDH
• UniProt Entry Name: SERA_HUMAN

NCBI Description

This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]

Uniprot Description

PHGDH: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation, and seizures. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Protein type: Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; EC 1.1.1.95; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: cytosol

Molecular Function: electron carrier activity; NAD binding; phosphoglycerate dehydrogenase activity

Biological Process: neural tube development; glial cell development; glycine metabolic process; gamma-aminobutyric acid metabolic process; glutamine metabolic process; L-serine biosynthetic process; spinal cord development; regulation of gene expression; amino acid biosynthetic process; brain development; taurine metabolic process; neurite development; threonine metabolic process

Disease: Phosphoglycerate Dehydrogenase Deficiency; Neu-laxova Syndrome 1