Mouse C. Perfringens Neuraminidase Monoclonal Antibody | anti-nanH antibody

Anti-C. Perfringens Neuraminidase Antibody, Mouse Monoclonal

Cat. Num. AAA8105612

• Reactivity: C.perfringens
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A
• Synonyms: C. Perfringens Neuraminidase; Monoclonal Antibody; Anti-C. Perfringens Neuraminidase Antibody; Mouse Monoclonal; Clostridium Perfringens Neuraminidase Antibody; Mouse MAb; Anti-NA Antibody; anti-nanH antibody

• Host: Mouse
• Reactivity: C.perfringens
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 2D7C1
• Specificity: C.perfringens C. Perfringens Neuraminidase.; No cross-reactivity in ELISA with E Coli cell lysate
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-nanH antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: This antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect Clostridium Perfringens Neuraminidase in WB. Using a DAB detection system, the detection limit for Clostridium Perfringens Neuraminidase is approximately 1ng/lane under non-reducing conditions and 2ng/lane under reducing conditions.; ELISA: 1:1000-1:2000
• Immunogen: Recombinant Clostridium Perfringens Neuraminidase Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Clostridium Perfringens Neuraminidase. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-nanH antibody

Clostridium perfringens/C. perfringens (formerly known as C. welchii) is a Gram-positive, rod-shaped, anaerobic, spore-forming bacterium of the genus Clostridium. C. perfringens is ubiquitous in nature and can be found as a normal component of decaying vegetation, marine sediment, the intestinal tract of humans and other vertebrates, insects, and soil. C. perfringens is commonly encountered in infections as a benign component of the normal flora. In this case, its role in disease is minor. Infections due to C. perfringens show evidence of tissue necrosis, bacteremia, emphysematous cholecystitis, and gas gangrene, which is also known as clostridial myonecrosis. NA, also called sialidases, specifically catalyze the hydrolysis removal of terminal sialic acid residues from viral and cellular glycoconjugates. C. Perfringens neuraminidase catalyzes the hydrolysis of alpha-(2->3)-, alpha-(2->6)-, glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates, but has little activity against the alpha2-8 glycosidic linkages. The function of the neuraminidase is to release sialic acids for use as carbon and energy sources for the non-pathogenic bacterium, while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors

NCBI and Uniprot Product Information

• NCBI GI #: 127825
• UniProt Accession #: P10481
• Molecular Weight: 42,813 Da
• NCBI Official Full Name: Sialidase
• UniProt Protein Name: Sialidase
• UniProt Gene Name: nanH
• UniProt Entry Name: NANH_CLOPF

Uniprot Description

Sialidases have been suggested to be pathogenic factors in microbial infections.