Immunofluorescence (IF)
(Immunofluorescence staining of ENPP-5 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human ENPP-5 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
Mouse anti-Human ENPP-5 Monoclonal Antibody | anti-ENPP5 antibody
Anti-ENPP-5 Antibody, Mouse Monoclonal
Gene Names
ENPP5; KIAA0879
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A
Synonyms
ENPP-5; Monoclonal Antibody; Anti-ENPP-5 Antibody; Mouse Monoclonal; ENPP-5 Antibody; Mouse MAb; ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative); Anti-NPP-5 Antibody; anti-ENPP5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
06
Specificity
Human ENPP-5
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ENPP5 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:20-1:100
Immunogen
Recombinant Human ENPP-5 Protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ENPP-5 (rh ENPP-5). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of ENPP-5 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human ENPP-5 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of ENPP-5 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human ENPP-5 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
Related Product Information for anti-ENPP5 antibody
ENPP5 is a member of the nucleotide pyrophosphatase/phosphodiesterase family (NPP). It is a family comprised of dimeric enzymes that catalyze the hydrolysis of phosphate diester bonds. There are seven isoforms in NPP family, some of which prefer nucleotide substrates, some of which prefer phospholipid substrates, and others of which prefer substrates that have not yet been determined. NPP also belongs to the alkaline phosphatase (AP) superfamily of enzymes and they are located in the cell membrane and hydrolyze extracellular phosphate diesters to affect a wide variety of biological processes. ENPP5 belongs to a group of nucleotidemetabolizing ectoenzymes, which regulate the availability of extracellular nucleotides. ENPP5 may play a role in neuronal cell communication. However, it lacks nucleotide pyrophosphatase and lysopholipase D activity. It may also be involved in neuronal cell communication. The amino acid sequence of human ENPP5 is 100%, 88%, and 82% identical to that of chimpanzee, dog and mouse/rat. ENPP5 functions in phospholipid metabolism.
Product Categories/Family for anti-ENPP5 antibody
References
Strausberg RL, et al. (2002) Generation and initial analysis of more than 15, 000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.Gerhard DS, et al. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7.Lamesch P, et al. (2007) hORFeome v3.1: a resource of human open reading frames representing over 10, 000 human genes. Genomics. 89(3):307-15.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,666 Da
NCBI Official Full Name
ectonucleotide pyrophosphatase/phosphodiesterase family member 5
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative)
NCBI Official Symbol
ENPP5
NCBI Official Synonym Symbols
KIAA0879
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 5; NPP-5; E-NPP 5; OTTHUMP00000016542; OTTHUMP00000016543; ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative function)
UniProt Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 5
Protein Family
UniProt Gene Name
ENPP5
UniProt Entry Name
ENPP5_HUMAN
NCBI Description
This gene encodes a type-I transmembrane glycoprotein. Studies in rat suggest the encoded protein may play a role in neuronal cell communications. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2014]
Uniprot Description
ENPP5: May play a role in neuronal cell communication. Lacks nucleotide pyrophosphatase and lysopholipase D activity. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Protein type: Membrane protein, integral; EC 3.1.-.-; Motility/polarity/chemotaxis; Hydrolase
Chromosomal Location of Human Ortholog: 6p21.1-p11.2
Cellular Component: plasma membrane; extracellular region; integral to membrane
Molecular Function: hydrolase activity; metal ion binding
Biological Process: metabolic process; cell communication
Similar Products
Product Notes
The ENPP5 enpp5 (Catalog #AAA8105374) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ENPP-5 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENPP-5 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100. Researchers should empirically determine the suitability of the ENPP5 enpp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENPP-5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
