Rabbit anti-Human ficolin 1/FCN1 Monoclonal Antibody | anti-FCN1 antibody

Recombinant Anti-ficolin 1/FCN1 Antibody, Rabbit Monoclonal

Cat. Num. AAA8104368

• Gene Names: FCN1; FCNM
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A
• Synonyms: ficolin 1/FCN1; Monoclonal Antibody; Recombinant Anti-ficolin 1/FCN1 Antibody; Rabbit Monoclonal; Ficolin-1/Ficolin-A/FCN1 Antibody; Rabbit MAb; ficolin 1; Anti-FCN1 Antibody; Anti-FCNA Antibody; Anti-FCNM Antibody; anti-FCN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 017
• Specificity: Human FCN1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-FCN1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-1:500
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human FCN1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human FCN1 (rh FCN1).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human FCN1 in human spleen (from 2 donors) with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to peripheral blood leukocytes.)

Immunohistochemistry (IHC)

(Immunochemical staining of human FCN1 in human placenta with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to peripheral blood leukocytes.)

Related Product Information for anti-FCN1 antibody

Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. The Ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. Three Ficolins have been identified in humans: L-Ficolin, H-Ficolin and M-Ficolin (also referred to as Ficolin-2, -3 and -1, respectively). They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Dysfunction or abnormal expressions of Ficolins may involved in the pathogenesis of human diseases including infectious and inflammatory diseases, autoimmune disease and clinical syndrome of preeclampsia. They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Upon recognition of the infectious agent, the Ficolins act through two distinct routes: initiate the lectin pathway of complement activation through attached serine proteases (MASPs), and a primitive opsonophagocytosis thus limiting the infection and concurrently orchestrating the subsequent adaptive clonal immune response. Ficolin-1 (FCN1) is predominantly expressed in the peripheral blood leukocytes.

References

Thiel S. (2007) Complement activating soluble pattern recognition molecules with collagen-like regions, mannan-binding lectin, ficolins and associated proteins. Mol Immunol. 44(16): 3875-88.Zhang XL, et al. (2008) Ficolins: structure, function and associated diseases. Adv Exp Med Biol. 632: 105-15.Garred P, et al. (2009) MBL2, FCN1, FCN2 and FCN3-The genes behind the initiation of the lectin pathway of complement. Mol Immunol. 46(14): 2737-44.

NCBI and Uniprot Product Information

• NCBI GI #: 8051584
• NCBI GeneID: 2219
• NCBI Accession #: NP_001994.2
• NCBI GenBank Nucleotide #: NM_002003.4
• UniProt Accession #: O00602; Q5VYV5; Q92596
• Molecular Weight: 35,078 Da
• NCBI Official Full Name: ficolin-1
• NCBI Official Synonym Full Names: ficolin 1
• NCBI Official Symbol: FCN1
• NCBI Official Synonym Symbols: FCNM
• NCBI Protein Information: ficolin-1
• UniProt Protein Name: Ficolin-1
• Protein Family: Ficolin
• UniProt Gene Name: FCN1
• UniProt Synonym Gene Names: FCNM
• UniProt Entry Name: FCN1_HUMAN

NCBI Description

The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct. Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity. [provided by RefSeq, Jul 2008]

Uniprot Description

FCN1: Complement-activating lectin and pattern recognition receptor. Binds GlcNAc. Binds preferentially to 9-O-acetylated 2- 6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. Belongs to the ficolin lectin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: collagen; extrinsic to external side of plasma membrane; extracellular region

Molecular Function: protein binding; G-protein-coupled receptor binding; metal ion binding; carbohydrate binding; pattern recognition receptor activity; sialic acid binding

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of virion penetration into host cell; cell surface pattern recognition receptor signaling pathway; innate immune response; complement activation, lectin pathway; recognition of apoptotic cell; complement activation