Rabbit anti-Human, Mouse CREG1 Polyclonal Antibody | anti-CREG1 antibody

CREG1, NT (CREG1, CREG, Protein CREG1, Cellular repressor of E1A-stimulated genes 1) (APC)

Cat. Num. AAA6288704

• Gene Names: CREG1; CREG
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CREG1; Polyclonal Antibody; NT (CREG1; CREG; Protein CREG1; Cellular repressor of E1A-stimulated genes 1) (APC); Cellular repressor of E1A-stimulated genes 1; anti-CREG1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CREG1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CREG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human CREG1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CREG1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CREG1 antibody detected the CREG1 protein (arrow).)

Western Blot (WB)

(CREG1 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the CREG1 antibody detected the CREG1 protein (arrow).)

Related Product Information for anti-CREG1 antibody

The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation.

Product Categories/Family for anti-CREG1 antibody

Antibodies; Transcription Factors

References

Han, Y., et al. J. Mol. Cell. Cardiol. 48(6):1225-1235(2010). Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009). Han, Y., et al. Exp. Cell Res. 315(19):3301-3311(2009). Bian, Z., et al. J. Cell. Mol. Med. 13(7):1302-1313(2009). Cheung, C.L., et al. Hum. Mol. Genet. 18(4):679-687(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503037
• NCBI GeneID: 8804
• NCBI Accession #: NP_003842.1
• NCBI GenBank Nucleotide #: NM_003851.2
• UniProt Accession #: O75629; Q8N9A3; B2RDD4
• Molecular Weight: 24,075 Da
• NCBI Official Full Name: protein CREG1
• NCBI Official Synonym Full Names: cellular repressor of E1A-stimulated genes 1
• NCBI Official Symbol: CREG1
• NCBI Official Synonym Symbols: CREG
• NCBI Protein Information: protein CREG1
• UniProt Protein Name: Protein CREG1
• Protein Family: Protein
• UniProt Gene Name: CREG1
• UniProt Synonym Gene Names: CREG
• UniProt Entry Name: CREG1_HUMAN

NCBI Description

The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

CREG1: May contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R. Belongs to the CREG family.

Protein type: Secreted, signal peptide; Secreted; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: transcription factor complex

Molecular Function: FMN binding; oxidoreductase activity; transcription factor binding; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; cell proliferation; regulation of growth; multicellular organismal development