Ficolin-1 / Ficolin-A / FCN1 Recombinant Protein | FCN1 recombinant protein

Recombinant Human Ficolin-1 / Ficolin-A / FCN1 Protein (His tag)

Cat. Num. AAA2546007

• Gene Names: FCN1; FCNM
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Ficolin-1 / Ficolin-A / FCN1; Recombinant Human Ficolin-1 / Ficolin-A / FCN1 Protein (His tag); FCN1; FCNM; FCN1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 326

• Application Notes: The secreted recombinant human FCN1 comprises 308 amino acids with a predicted molecular mass of 33.6 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 30
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FCN1 recombinant protein

Background: Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. The Ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. Three Ficolins have been identified in humans: L-Ficolin, H-Ficolin and M-Ficolin (also referred to as Ficolin-2, -3 and -1, respectively). They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Dysfunction or abnormal expressions of Ficolins may involved in the pathogenesis of human diseases including infectious and inflammatory diseases, autoimmune disease and clinical syndrome of preeclampsia. They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Upon recognition of the infectious agent, the Ficolins act through two distinct routes: initiate the lectin pathway of complement activation through attached serine proteases (MASPs), and a primitive opsonophagocytosis thus limiting the infection and concurrently orchestrating the subsequent adaptive clonal immune response. Ficolin-1 (FCN1) is predominantly expressed in the peripheral blood leukocytes.

Description: A DNA sequence encoding the human FCN1 (NP_001994.2) precursor (Met 1-Ala 326) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 8051584
• NCBI GeneID: 2219
• NCBI Accession #: NP_001994.2
• NCBI GenBank Nucleotide #: NM_002003.4
• UniProt Accession #: O00602; Q5VYV5; Q92596
• Molecular Weight: 35,078 Da
• NCBI Official Full Name: ficolin-1
• NCBI Official Synonym Full Names: ficolin 1
• NCBI Official Symbol: FCN1
• NCBI Official Synonym Symbols: FCNM
• NCBI Protein Information: ficolin-1
• UniProt Protein Name: Ficolin-1
• Protein Family: Ficolin
• UniProt Gene Name: FCN1
• UniProt Synonym Gene Names: FCNM

NCBI Description

The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct. Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity. [provided by RefSeq, Jul 2008]

Uniprot Description

FCN1: Complement-activating lectin and pattern recognition receptor. Binds GlcNAc. Binds preferentially to 9-O-acetylated 2- 6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. Belongs to the ficolin lectin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: extracellular region; extrinsic to external side of plasma membrane

Molecular Function: G-protein-coupled receptor binding; pattern recognition receptor activity; protein binding; serine-type endopeptidase activity; sialic acid binding

Biological Process: cell surface pattern recognition receptor signaling pathway; complement activation; complement activation, lectin pathway; G-protein coupled receptor protein signaling pathway; negative regulation of virion penetration into host cell; recognition of apoptotic cell