Mouse anti-Human Urokinase/uPA Monoclonal Antibody | anti-PLAU antibody

Anti-Urokinase/uPA Antibody (HRP), Mouse Monoclonal

Cat. Num. AAA8104020

• Gene Names: PLAU; ATF; QPD; UPA; URK; u-PA; BDPLT5
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A
• Synonyms: Urokinase/uPA; Monoclonal Antibody; Anti-Urokinase/uPA Antibody (HRP); Mouse Monoclonal; Urokinase/uPA/PLAU Antibody (HRP); Mouse MAb; plasminogen activator; urokinase; Anti-ATF Antibody; Anti-BDPLT5 Antibody; Anti-QPD Antibody; Anti-u-PA Antibody; Anti-UPA Antibody; Anti-URK Antibody; anti-PLAU antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: 05
• Specificity: Human Urokinase/uPA/PLAU
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PLAU antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-1ug/ml
• Immunogen: Recombinant Human Urokinase/PLAU Protein
• Conjugation: HRP
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Urokinase/PLAU (rh Urokinase/PLAU). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and conjugated with horseradish-peroxidase (HRP).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Protected from prolonged exposure to light. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-PLAU antibody

Plasminogen activator, urokinase, also known as PLAU and uPA, is a serine protease which converts plasminogen to plasmin, a broad-spectrum protease active on extracellular matrix (ECM) components. It is involved in complement activation, cell migration, wound healing, and generation of localized extracellular proteolysis during tissue remodelling, pro-hormone conversion, carcinogenesis and neoplasia. Like many components of the blood coagulation, fibrinolytic and complement cascades, uPA has a modular structure, including three conserved domains: a growth factor-like domain (GFD, residues 1-49), a kringle domain (residues 50-131), linked by an interdomain linker or "connecting peptide" (CP, residues 132-158) to the serine protease domain (residues 159-411). uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction). The role of uPA is not only linked to its action as an enzyme. In fact, the mere binding of uPA on the cell surface also brings about two events that broaden the spectrum of its biological functions: (1) a conformational change of the receptor, which, in turn, affects its interaction with other proteins; (2) a signal transduction which modulates the expression of apoptosis-related genes. Besides its applications as a thrombolytic agent and as a prognostic marker for tumors, uPA may provide the basis for other therapies, as the structure of the receptor-binding domain of uPA has become a model for the design of anti-cancer molecules. Because of the causal involvment of uPA in cancer invasion and metastasis, the blockade of uPA interactions and activity with specific inhibitors is of interest for novel strategies in cancer therapy.

References

Crippa MP. (2007) Urokinase-type plasminogen activator. Int J Biochem Cell Biol. 39(4): 690-4.Kunamneni A, et al. (2008) Urokinase-a very popular cardiovascular agent. Recent Pat Cardiovasc Drug Discov. 3(1): 45-58.Vincenza Carriero M, et al. (2009) Structure, function and antagonists of urokinase-type plasminogen activator. Front Biosci. 14: 3782-94.Xu J, et al. (2010) Association of putative functional variants in the PLAU gene and the PLAUR gene with myocardial infarction. Clin Sci (Lond). 119(8): 353-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4505863
• NCBI GeneID: 5328
• NCBI Accession #: NP_002649.1
• NCBI GenBank Nucleotide #: NM_002658.3
• Molecular Weight: 46,908 Da
• NCBI Official Full Name: urokinase-type plasminogen activator isoform 1 preproprotein
• NCBI Official Synonym Full Names: plasminogen activator, urokinase
• NCBI Official Symbol: PLAU
• NCBI Official Synonym Symbols: ATF; QPD; UPA; URK; u-PA; BDPLT5
• NCBI Protein Information: urokinase-type plasminogen activator; U-plasminogen activator; plasminogen activator, urinary
• Protein Family: Urokinase-type plasminogen activator