Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Kidney Tissue; Lane3: Human Hela Cells; Lane4: Human 293T Cells.)
Rabbit anti-Human Radixin Polyclonal Antibody | anti-RDX antibody
Radixin (RDX) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Kidney Tissue; Lane3: Human Hela Cells; Lane4: Human 293T Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Kidney Tissue; Lane3: Human Hela Cells; Lane4: Human 293T Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant RDX, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant RDX, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
NCBI and Uniprot Product Information
NCBI Description
Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Uniprot Description
Radixin: a cytoskeletal protein of the ERM family which includes ezrin and moiesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. May play a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11q22.3
Cellular Component: apical plasma membrane; cell periphery; cell-cell adherens junction; cleavage furrow; cortical actin cytoskeleton; extracellular exosome; extracellular space; filopodium; focal adhesion; lamellipodium; microvillus; midbody; myelin sheath; plasma membrane; ruffle; stereocilium; T-tubule
Molecular Function: actin binding; ATPase binding; cadherin binding; protein binding; protein domain specific binding; protein homodimerization activity; protein kinase A binding; RNA binding
Biological Process: apical protein localization; barbed-end actin filament capping; establishment of protein localization; microvillus assembly; negative regulation of cell size; negative regulation of homotypic cell-cell adhesion; positive regulation of cell migration; positive regulation of early endosome to late endosome transport; positive regulation of G1/S transition of mitotic cell cycle; positive regulation of gene expression; protein kinase A signaling; protein localization to plasma membrane; regulation of actin filament bundle assembly; regulation of cell shape; regulation of cell size; regulation of GTPase activity; regulation of organelle assembly; regulation of Rap protein signal transduction; regulation of ruffle assembly
Disease: Deafness, Autosomal Recessive 24
Research Articles on RDX
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Product Notes
The RDX rdx (Catalog #AAA2032621) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Radixin (RDX) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Radixin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the RDX rdx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Radixin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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