Rabbit RPS6 Polyclonal Antibody | anti-RPS6 antibody

Anti-RPS6 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103438

• Gene Names: Rps6; S6R
• Reactivity: Mouse, Rat, Human; Predicted to React with: Bovin, Cynomolgus (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: RPS6; Polyclonal Antibody; Anti-RPS6 Antibody; Rabbit Polyclonal; RPS6 Antibody; Rabbit PAb; Antigen Affinity Purified; ribosomal protein S6; Anti-S6R Antibody; anti-RPS6 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human; Predicted to React with: Bovin, Cynomolgus (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse RPS6
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-RPS6 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:200-1:1000; ICC/IF: 1:100-1:500
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse RPS6
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse RPS6, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining RPS6 in mouse brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining RPS6 in rat brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining RPS6 in human brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of RPS6 in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RPS6 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-RPS6 antibody

Ribosomal protein S6 (rpS6), a component of the 40S ribosomal subunit, is involved in multiple cellular bioactivities. RPS6 is a downstream substrate of PI3K/Akt/mTOR/ kinase and MAPK kinase pathways and its dephosphorylation is also triggered by DNA double-strand breaks. The ribosomal protein S6 (RPS6) is a downstream component of the signaling mediated by the target of rapamycin (TOR) kinase that acts as a central regulator of the key metabolic processes, such as protein translation and ribosome biogenesis, in response to various environmental cues. mTORC1/rpS6 signaling complex promoted Sertoli blood-testis barrier (BTB) remodeling by perturbing Sertoli cell-cell adhesion site known as the basal ectoplasmic specialization (ES). mTORC1/rpS6 complex also promoted disruption of spermatid adhesion at the Sertoli-spermatid interface called the apical ES.

Product Categories/Family for anti-RPS6 antibody

AMPK Signaling Pathway

References

Wu S, et al. (2019) Mtorc1/rps6 and spermatogenic function in the testis-insights from the adjudin model. Reprod Toxicol 89 54-66.Son O, et al. (2015) Identification of nucleosome assembly protein 1 (nap1) as an interacting partner of plant ribosomal protein s6 (rps6) and a positive regulator of rdna transcription. Biochem Biophys Res Commun 465 (2): 200-205.Grundy M, et al. (2018) Early changes in rps6 phosphorylation and bh3 profiling predict response to chemotherapy in aml cells. PLoS One 13 (5): e0196805.

NCBI and Uniprot Product Information

• NCBI GI #: 6677809
• NCBI GeneID: 20104
• NCBI Accession #: NP_033122.1
• NCBI GenBank Nucleotide #: NM_009096.3
• UniProt Accession #: P62754; P08227; P10660
• Molecular Weight: 28
• NCBI Official Full Name: 40S ribosomal protein S6
• NCBI Official Synonym Full Names: ribosomal protein S6
• NCBI Official Symbol: Rps6
• NCBI Official Synonym Symbols: S6R
• NCBI Protein Information: 40S ribosomal protein S6; phosphoprotein NP33
• UniProt Protein Name: 40S ribosomal protein S6
• Protein Family: 30S ribosomal protein
• UniProt Gene Name: Rps6
• UniProt Entry Name: RS6_MOUSE

Uniprot Description

Function: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

Post-translational modification: Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex. Ref.5 Ref.6

Sequence similarities: Belongs to the ribosomal protein S6e family.