Focal adhesion kinase 1 Recombinant Protein | PTK2 recombinant protein

Recombinant Chicken Focal adhesion kinase 1

Cat. Num. AAA1265406

• Gene Names: PTK2; FAK; FRNK; p125FAK
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Focal adhesion kinase 1; Recombinant Chicken Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; FRNK; p41/p43; Protein-tyrosine kinase 2; p125; FAKpp; 125FAK; PTK2 recombinant protein

• Host: E Coli
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 376-683
• Sequence: LANNEKQGVRSHTVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPAMAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKFSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSATDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNND
• Sequence Length: 1053

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-Page

Related Product Information for PTK2 recombinant protein

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development, embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), ephrin receptors, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Regulates P53/TP53 activity and stability. Phosphorylates SRC; this increases SRC kinase activity. Isoform 2 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling.

Product Categories/Family for PTK2 recombinant protein

Cancer

References

pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.Schaller M.D., Borgman C.A., Cobb B.S., Vines R.R., Reynolds A.B., Parsons J.T.Proc. Natl. Acad. Sci. U.S.A. 89:5192-5196(1992) Chicken and mouse focal adhesion kinases are similar in structure at their amino termini.Devor B.B., Zhang X., Patel S.K., Polte T.R., Hanks S.K.Biochem. Biophys. Res. Commun. 190:1084-1089(1993) Autonomous expression of a noncatalytic domain of the focal adhesion-associated protein tyrosine kinase pp125FAK.Schaller M.D., Borgman C.A., Parsons J.T.Mol. Cell. Biol. 13:785-791(1993) An SH3 domain-containing GTPase-activating protein for Rho and Cdc42 associates with focal adhesion kinase.Hildebrand J.D., Taylor J.M., Parsons J.T.Mol. Cell. Biol. 16:3169-3178(1996) Requirement of phosphatidylinositol 3-kinase in focal adhesion kinase-promoted cell migration.Reiske H.R., Kao S.C., Cary L.A., Guan J.L., Lai J.F., Chen H.C.J. Biol. Chem. 274:12361-12366(1999) Association of Grb7 with phosphoinositides and its role in the regulation of cell migration.Shen T.L., Han D.C., Guan J.L.J. Biol. Chem. 277:29069-29077(2002) Tyr-863 phosphorylation enhances focal adhesion kinase autophosphorylation at Tyr-397.Leu T.H., Maa M.C.Oncogene 21:6992-7000(2002) Focal adhesion kinase in netrin-1 signaling.Ren X.R., Ming G.L., Xie Y., Hong Y., Sun D.M., Zhao Z.Q., Feng Z., Wang Q., Shim S., Chen Z.F., Song H.J., Mei L., Xiong W.C.Nat. Neurosci. 7:1204-1212(2004) Activation of FAK and Src are receptor-proximal events required for netrin signaling.Li W., Lee J., Vikis H.G., Lee S.H., Liu G., Aurandt J., Shen T.L., Fearon E.R., Guan J.L., Han M., Rao Y., Hong K., Guan K.L.Nat. Neurosci. 7:1213-1221(2004) Netrin requires focal adhesion kinase and Src family kinases for axon outgrowth and attraction.Liu G., Beggs H., Jurgensen C., Park H.T., Tang H., Gorski J., Jones K.R., Reichardt L.F., Wu J., Rao Y.Nat. Neurosci. 7:1222-1232(2004) Focal adhesion kinase controls actin assembly via a FERM-mediated interaction with the Arp2/3 complex.Serrels B., Serrels A., Brunton V.G., Holt M., McLean G.W., Gray C.H., Jones G.E., Frame M.C.Nat. Cell Biol. 9:1046-1056(2007) FRNK inhibition of focal adhesion kinase-dependent signaling and migration in vascular smooth muscle cells.Koshman Y.E., Kim T., Chu M., Engman S.J., Iyengar R., Robia S.L., Samarel A.M.Arterioscler. Thromb. Vasc. Biol. 30:2226-2233(2010) Focal adhesion kinase-related nonkinase inhibits vascular smooth muscle cell invasion by focal adhesion targeting, tyrosine 168 phosphorylation, and competition for p130Cas Binding.Koshman Y.E., Chu M., Engman S.J., Kim T., Iyengar R., Robia S.L., Samarel A.M.Arterioscler. Thromb. Vasc. Biol. 31:2432-2440(2011) Serine-910 phosphorylation of focal adhesion kinase is critical for sarcomere reorganization in cardiomyocyte hypertrophy.Chu M., Iyengar R., Koshman Y.E., Kim T., Russell B., Martin J.L., Heroux A.L., Robia S.L., Samarel A.M.Cardiovasc. Res. 92:409-419(2011) Structural insight into the mechanisms of targeting and signaling of focal adhesion kinase.Liu G., Guibao C.D., Zheng J.Mol. Cell. Biol. 22:2751-2760(2002) NMR solution structure of the focal adhesion targeting domain of focal adhesion kinase in complex with a paxillin LD peptide evidence for a two-site binding model.Gao G., Prutzman K.C., King M.L., Scheswohl D.M., DeRose E.F., London R.E., Schaller M.D., Campbell S.L.J. Biol. Chem. 279:8441-8451(2004) The focal adhesion targeting domain of focal adhesion kinase contains a hinge region that modulates tyrosine 926 phosphorylation.Prutzman K.C., Gao G., King M.L., Iyer V.V., Mueller G.A., Schaller M.D., Campbell S.L.Structure 12:881-891(2004) Crystal structure of the FERM domain of focal adhesion kinase.Ceccarelli D.F., Song H.K., Poy F., Schaller M.D., Eck M.J.J. Biol. Chem. 281:252-259(2006) Structural basis for the autoinhibition of focal adhesion kinase.Lietha D., Cai X., Ceccarelli D.F., Li Y., Schaller M.D., Eck M.J.Cell 129:1177-1187(2007) Crystal structures of the FAK kinase in complex with TAE226 and related bis-anilino pyrimidine inhibitors reveal a helical DFG conformation.Lietha D., Eck M.J.PLoS ONE 3:E3800-E3800(2008)

NCBI and Uniprot Product Information

• NCBI GI #: 45382167
• NCBI GeneID: 396416
• NCBI Accession #: NP_990766.1
• NCBI GenBank Nucleotide #: NM_205435.1
• UniProt Accession #: Q00944
• Molecular Weight: 62.6kD
• NCBI Official Full Name: focal adhesion kinase 1
• NCBI Official Synonym Full Names: protein tyrosine kinase 2
• NCBI Official Symbol: PTK2
• NCBI Official Synonym Symbols: FAK; FRNK; p125FAK
• NCBI Protein Information: focal adhesion kinase 1
• UniProt Protein Name: Focal adhesion kinase 1
• Protein Family: Focal adhesion kinase
• UniProt Gene Name: PTK2
• UniProt Synonym Gene Names: FAK; FAK1; FADK 1; FRNK; p41/p43FRNK
• UniProt Entry Name: FAK1_CHICK

Uniprot Description

FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Fak family

Cellular Component: cell; cytoplasm; extrinsic to internal side of plasma membrane; focal adhesion; microtubule organizing center; nucleus; perinuclear region of cytoplasm; sarcolemma

Molecular Function: ATP binding; calcium-dependent cysteine-type endopeptidase activity; identical protein binding; non-membrane spanning protein tyrosine kinase activity; protease binding; protein binding; protein-tyrosine kinase activity; receptor binding; signal transducer activity

Biological Process: actin filament organization; angiogenesis; angiogenesis involved in wound healing; cell adhesion; cell differentiation; cytoskeleton organization and biogenesis; epidermal growth factor receptor signaling pathway; innate immune response; negative regulation of apoptosis; negative regulation of protein amino acid autophosphorylation; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of focal adhesion formation; positive regulation of protein binding; protein amino acid autophosphorylation; proteolysis; pyramidal neuron migration; regulation of cell adhesion; regulation of cell proliferation; response to pH; signal complex assembly