Rabbit RPS6 Polyclonal Antibody | anti-RPS6 antibody

Anti-RPS6 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100584

• Gene Names: RPS6; S6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: RPS6; Polyclonal Antibody; Anti-RPS6 Antibody; Rabbit Polyclonal; RPS6 Antibody; Rabbit PAb; Antigen Affinity Purified; ribosomal protein S6; Anti-S6 Antibody; anti-RPS6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RPS6
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-RPS6 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:1000-1:5000; IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human RPS6
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human RPS6, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-RPS6 rabbit polyclonal antibody at 1:2000 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:32 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining RPS6 in human colon carcinoma with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining RPS6 in human pancreas with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining RPS6 in mouse pancreas with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining RPS6 in rat pancreas with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-RPS6 antibody

Ribosomal protein S6 (rpS6), a component of the 40S ribosomal subunit, is involved in multiple cellular bioactivities. RPS6 is a downstream substrate of PI3K/Akt/mTOR/ kinase and MAPK kinase pathways and its dephosphorylation is also triggered by DNA double-strand breaks. The ribosomal protein S6 (RPS6) is a downstream component of the signaling mediated by the target of rapamycin (TOR) kinase that acts as a central regulator of the key metabolic processes, such as protein translation and ribosome biogenesis, in response to various environmental cues. mTORC1/rpS6 signaling complex promoted Sertoli blood-testis barrier (BTB) remodeling by perturbing Sertoli cell-cell adhesion site known as the basal ectoplasmic specialization (ES). mTORC1/rpS6 complex also promoted disruption of spermatid adhesion at the Sertoli-spermatid interface called the apical ES.

Product Categories/Family for anti-RPS6 antibody

AMPK Signaling Pathway

References

Wu S, et al. (2019) Mtorc1/rps6 and spermatogenic function in the testis-insights from the adjudin model. Reprod Toxicol 89 54-66.Son O, et al. (2015) Identification of nucleosome assembly protein 1 (nap1) as an interacting partner of plant ribosomal protein s6 (rps6) and a positive regulator of rdna transcription. Biochem Biophys Res Commun 465 (2): 200-205.Grundy M, et al. (2018) Early changes in rps6 phosphorylation and bh3 profiling predict response to chemotherapy in aml cells. PLoS One 13 (5): e0196805.

NCBI and Uniprot Product Information

• NCBI GI #: 17158044
• NCBI GeneID: 6194
• NCBI Accession #: NP_001001.2
• NCBI GenBank Nucleotide #: NM_001010.2
• UniProt Accession #: P62753; P08227; P10660; Q4VBY7; Q8N6Z7
• Molecular Weight: 28,681 Da
• NCBI Official Full Name: 40S ribosomal protein S6
• NCBI Official Synonym Full Names: ribosomal protein S6
• NCBI Official Symbol: RPS6
• NCBI Official Synonym Symbols: S6
• NCBI Protein Information: 40S ribosomal protein S6; phosphoprotein NP33
• UniProt Protein Name: 40S ribosomal protein S6
• Protein Family: 30S ribosomal protein
• UniProt Gene Name: RPS6
• UniProt Entry Name: RS6_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

S6: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Belongs to the ribosomal protein S6e family.

Protein type: Translation

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: polysome; small ribosomal subunit; membrane; perinuclear region of cytoplasm; dendrite; cytoplasm; nucleolus; nucleus; ribonucleoprotein complex; cytosol

Molecular Function: protein binding; structural constituent of ribosome; protein kinase binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; erythrocyte development; TOR signaling pathway; translation; viral reproduction; positive regulation of apoptosis; T cell differentiation in the thymus; gastrulation; translational termination; viral infectious cycle; glucose homeostasis; oogenesis stage; T cell proliferation during immune response; translational initiation; ribosomal small subunit assembly and maintenance; viral transcription; activated T cell apoptosis; placenta development; mitosis; mitotic cell cycle checkpoint; ribosomal small subunit biogenesis and assembly; translational elongation; cellular protein metabolic process; mRNA catabolic process, nonsense-mediated decay; insulin receptor signaling pathway; gene expression; rRNA processing; G1/S transition of mitotic cell cycle; negative regulation of apoptosis