Rabbit anti-Mouse MMP-3 Polyclonal Antibody | anti-MMP3 antibody

Anti-MMP-3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103370

• Gene Names: Mmp3; SLN1; Str1; SLN-1; STR-1; Stmy1
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: MMP-3; Polyclonal Antibody; Anti-MMP-3 Antibody; Rabbit Polyclonal; MMP-3 Antibody; Rabbit PAb; Antigen Affinity Purified; matrix metallopeptidase 3; Anti-SLN-1 Antibody; Anti-SLN1 Antibody; Anti-Stmy1 Antibody; Anti-STR-1 Antibody; Anti-Str1 Antibody; anti-MMP3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse MMP-3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MMP3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse MMP-3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse MMP-3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MMP3 rabbit polyclonal antibody at 1:500 dilution Lane A: H1299 Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: Raji Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:53 kDa Observed band size:58 kDa)

Related Product Information for anti-MMP3 antibody

Matrix metallopeptidase 3 (abbreviated as MMP3) is also known as stromelysin 1 and progelatinase. MMP3 is a member of the matrix metalloproteinase (MMP) family whose members are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes including arthritis and metastasis. As a secreted zinc-dependent endopeptidase, MMP3 exerts its functions mainly in the extracellular matrix. This protein is activated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMP3 plays a central role in degrading collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. Also, MMP3 can active other MMPs such as MMP1, MMP7, and MMP9, rendering MMP3 crucial in connective tissue remodeling. Dysregulation of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis, and cancer. Synthetic or natural inhibitors of MMPs result in inhibition of metastasis, while up-regulation of MMPs led to enhanced cancer cell invasion.

Product Categories/Family for anti-MMP3 antibody

IL17 signaling pathway

References

Sternlicht MD, et al. 1999, The stromal proteinase MMP3/stromelysin-1 promotes mammary carcinogenesis. Cell. 98(2): 137-46.Yoon S, et al. (1999) Genetic analysis of MMP3, MMP9, and PAI-1 in Finnish patients with abdominal aortic or intracranial aneurysms. Biochem Biophys Res Commun. 265(2): 563-8.Biondi ML, et al. (2000) MMP1 and MMP3 polymorphisms in promoter regions and cancer. Clin Chem. 46(12): 2023-4.

NCBI and Uniprot Product Information

• NCBI GI #: 461769
• NCBI GeneID: 17392
• UniProt Accession #: P28862
• Molecular Weight: 53,845 Da
• NCBI Official Full Name: Stromelysin-1
• NCBI Official Synonym Full Names: matrix metallopeptidase 3
• NCBI Official Symbol: Mmp3
• NCBI Official Synonym Symbols: SLN1; Str1; SLN-1; STR-1; Stmy1
• NCBI Protein Information: stromelysin-1; SL-1; EMS-2; MMP-3; transin-1; progelatinase; stromelysin 1; matrix metalloproteinase 3; matrix metalloproteinase-3
• UniProt Protein Name: Stromelysin-1
• Protein Family: Stromelysin
• UniProt Gene Name: Mmp3
• UniProt Synonym Gene Names: SL-1; MMP-3
• UniProt Entry Name: MMP3_MOUSE

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that is involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded protein is activated by the removal of an N-temrinal activation peptide to generate a zinc-dependent endopeptidase with a broad range of substrates such as proteoglycans, laminin, fibronectin, elastin, and collagens. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Aug 2015]

Uniprot Description

MMP3: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Defects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6). A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions. Belongs to the peptidase M10A family.

Protein type: Motility/polarity/chemotaxis; Secreted; Protease; Secreted, signal peptide; EC 3.4.24.17

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; protein complex; mitochondrion; dendrite; extracellular region; cytosol; nucleus

Molecular Function: peptidase activity; hydrolase activity; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; metal ion binding; endopeptidase activity; protein complex binding; calcium ion binding

Biological Process: positive regulation of protein oligomerization; collagen catabolic process; negative regulation of protein kinase B signaling cascade; protein catabolic process; proteolysis; regulation of cell migration; positive regulation of cell migration