Mouse MMP-3 ELISA Kit | MMP3 elisa kit

Mouse MMP-3 PicoKine ELISA Kit

Cat. Num. AAA176003

• Gene Names: Mmp3; SLN1; Str1; SLN-1; STR-1; Stmy1
• Reactivity: Mouse
• Synonyms: MMP-3; Mouse MMP-3 PicoKine ELISA Kit; Stromelysin-1; matrix metallopeptidase 3 (stromelysin 1; progelatinase); CHDS6; Matrix metalloproteinase 3; Matrix metalloproteinase 3 preproprotein; Matrix metalloproteinase-3; MGC126102; MGC126103; MGC126104; MMP 3; MMP3; MMP3_HUMAN; Progelatinase; Proteoglycanase; SL 1; SL-1; SL1; STMY; STMY1; STR1; Stromelisin 1; Stromelysin 1; Stromelysin 1 progelatinase; Transin 1; Transin-1; MMP3 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse MMP3
• Sequence Length: 477

• Samples: Cell culture supernates, serum and plasma (heparin)
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP3 elisa kit

Principle of the Assay: The Picokine™ Mouse MMP3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse MMP3 with a 96-well strip plate that is pre-coated with antibody specific for MMP3. The detection antibody is a biotinylated antibody specific for MMP3. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse MMP3 with immunogen: Expression system for standard: NSO; Immunogen sequence: Y18-C477. The kit is analytically validated with ready to use reagents. To measure Mouse MMP3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse MMP3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse MMP3 in the sample.!!Background/Introduction: Matrix metalloproteinase-3(MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase(MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.

NCBI and Uniprot Product Information

• NCBI GI #: 461769
• NCBI GeneID: 17392
• UniProt Accession #: P28862
• Molecular Weight: 53,845 Da
• NCBI Official Full Name: Stromelysin-1
• NCBI Official Synonym Full Names: matrix metallopeptidase 3
• NCBI Official Symbol: Mmp3
• NCBI Official Synonym Symbols: SLN1; Str1; SLN-1; STR-1; Stmy1
• NCBI Protein Information: stromelysin-1; SL-1; EMS-2; MMP-3; transin-1; progelatinase; stromelysin 1; matrix metalloproteinase 3; matrix metalloproteinase-3
• UniProt Protein Name: Stromelysin-1
• Protein Family: Stromelysin
• UniProt Gene Name: Mmp3
• UniProt Synonym Gene Names: SL-1; MMP-3
• UniProt Entry Name: MMP3_MOUSE

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that is involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded protein is activated by the removal of an N-temrinal activation peptide to generate a zinc-dependent endopeptidase with a broad range of substrates such as proteoglycans, laminin, fibronectin, elastin, and collagens. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Aug 2015]

Uniprot Description

MMP3: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Defects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6). A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions. Belongs to the peptidase M10A family.

Protein type: Motility/polarity/chemotaxis; Secreted; Protease; Secreted, signal peptide; EC 3.4.24.17

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; protein complex; mitochondrion; dendrite; extracellular region; cytosol; nucleus

Molecular Function: peptidase activity; hydrolase activity; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; metal ion binding; endopeptidase activity; protein complex binding; calcium ion binding

Biological Process: positive regulation of protein oligomerization; collagen catabolic process; negative regulation of protein kinase B signaling cascade; protein catabolic process; proteolysis; regulation of cell migration; positive regulation of cell migration