Mouse anti-Human TREM-1 Monoclonal Antibody | anti-TREM1 antibody

Anti-TREM-1 Antibody, Mouse Monoclonal

Cat. Num. AAA8103047

• Gene Names: TREM1; CD354; TREM-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: TREM-1; Monoclonal Antibody; Anti-TREM-1 Antibody; Mouse Monoclonal; TREM-1/TREM1 Antibody; Mouse MAb; triggering receptor expressed on myeloid cells 1; Anti-CD354 Antibody; anti-TREM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 02
• Specificity: Human TREM-1/TREM1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TREM1 antibody: Flow Cytometry (FC/FACS/FCM)
• Application Notes: FC/FACS/FCM: 1:25-1:100
• Immunogen: Recombinant Human TREM-1/TREM1 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TREM-1/TREM1 (rh TREM-1/TREM1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human TREM-1(CD354) expression on human whole blood granulocytes. Cells were stained with purified anti-Human TREM-1(CD354), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-TREM1 antibody

TREM1 (triggering receptor expressed on myeloid cells) is a type I transmembrane protein with a single Ig-like domain, and is selectively expressed on blood neutrophils and a subset of monocytes. As a member of the growing family of receptors related to NK cell receptors, TREM1 activates downstream signaling events with the help of an adapter protein called DAP12. Expression of TREM1 is up-regulated by bacterial LPS, a ligand for TLR4, as well as lipoteichoic acid. Although its natural ligand has not been identified, engagement of TREM1 with agonist mAbs triggers secretion of the proinflammatory cytokines TNF-alpha and IL-1beta, as well as chemokines such as IL-8 and monocyte chemoattractant protein (MCP)-1. Intracellularly, TREM1 induces Ca2+ mobilization and tyrosine phosphorylation of extracellular signal-related kinase 1 (ERK1), ERK2 and phospholipase C-gamma. In an animal model of LPS-induced septic shock, blockade of TREM1 signaling inhibited hyperresponsiveness and death. Thus, it has been demonstrated that TREM1 performs a critical function in immune responses involved in host defense against microbial challenges, and is suggested to be a potential therapeutic target for septic shock.

References

Bouchon, A. et al., 2000, J. Immunol. 164: 4991-4995. Bouchon, A. et al., 2001, Nature. 410: 1103-1107. Bleharski, J.R. et al., 2003, J. Immunol. 170: 3812-3818.

NCBI and Uniprot Product Information

• NCBI GI #: 8924262
• NCBI GeneID: 54210
• NCBI Accession #: NP_061113.1
• NCBI GenBank Nucleotide #: NM_018643.3
• UniProt Accession #: Q9NP99; Q53FL8; Q5T2C9; Q86YU1; B4DWG2; K7EJW1
• Molecular Weight: 25,574 Da
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 1 isoform 1
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 1
• NCBI Official Symbol: TREM1
• NCBI Official Synonym Symbols: CD354; TREM-1
• NCBI Protein Information: triggering receptor expressed on myeloid cells 1
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 1
• Protein Family: Triggering receptor expressed on myeloid cells
• UniProt Gene Name: TREM1
• UniProt Synonym Gene Names: TREM-1
• UniProt Entry Name: TREM1_HUMAN

NCBI Description

This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

TREM1: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: extracellular region; integral to membrane; plasma membrane

Molecular Function: receptor activity

Biological Process: neutrophil chemotaxis; innate immune response; cytokine secretion during immune response; blood coagulation; leukocyte migration; chemokine metabolic process; humoral immune response