Rabbit anti-Human actinin alpha 1/ACTN1 Polyclonal Antibody | anti-ACTN1 antibody

Anti-actinin alpha 1/ACTN1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102933

• Gene Names: ACTN1; BDPLT15
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: actinin alpha 1/ACTN1; Polyclonal Antibody; Anti-actinin alpha 1/ACTN1 Antibody; Rabbit Polyclonal; actinin alpha 1/ACTN1 Antibody; Rabbit PAb; Antigen Affinity Purified; actinin; alpha 1; Anti-BDPLT15 Antibody; anti-ACTN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human actinin alpha 1/ACTN1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ACTN1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-PImmunochemical staining of human ACTN1 in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human actinin alpha 1/ACTN1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human actinin alpha 1/ACTN1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ACTN1 rabbit polyclonal antibody at 1:500 dilution Lane A: NIH3T3 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:103 kDa Observed band size:103 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human ACTN1 in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human ACTN1 in human liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-ACTN1 antibody

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. ACTN1-related macrothrombocytopenia. Mutations in the gene ACTN1 cause mild macrothrombocytopenia characterized by elevated mean platelet volume and elevated immature platelet fraction, and low bleeding tendency. Its transmission is autosomal dominant. Molecular identification is made by sequencing the ACTN1 gene. ACTN1 was also found to act as an effector for the isoform EFA6B (also known as PSD4) in the human mammary tumoral MCF7 cell line. EFA6B restored the glandular morphology of this tumoral cell line in an ACTN1-dependent manner. Thus, we identified new regulators of cyst luminogenesis essential for the proper maturation of a newly-formed lumen into a single central lumen.

Product Categories/Family for anti-ACTN1 antibody

T Cell Receptor Signaling Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 4501891
• NCBI GeneID: 87
• NCBI Accession #: NP_001093.1
• NCBI GenBank Nucleotide #: NM_001102.3
• UniProt Accession #: P12814; Q1HE25; Q9BTN1; B3V8S3; B4DHH3
• Molecular Weight: 103,058 Da
• NCBI Official Full Name: alpha-actinin-1 isoform b
• NCBI Official Synonym Full Names: actinin, alpha 1
• NCBI Official Symbol: ACTN1
• NCBI Official Synonym Symbols: BDPLT15
• NCBI Protein Information: alpha-actinin-1; actinin 1 smooth muscle; non-muscle alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform
• UniProt Protein Name: Alpha-actinin-1
• Protein Family: Alpha-actinin
• UniProt Gene Name: ACTN1
• UniProt Entry Name: ACTN1_HUMAN

NCBI Description

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q24|14q22-q24

Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; dendritic spine; extracellular region; fascia adherens; pseudopodium; cytosol; Z disc; ruffle; cell projection; cytoplasm; plasma membrane; stress fiber; nucleus

Molecular Function: integrin binding; actin filament binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; double-stranded RNA binding; calcium ion binding; vinculin binding

Biological Process: actin crosslink formation; regulation of apoptosis; focal adhesion formation; actin filament bundle formation; platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; negative regulation of cell motility; blood coagulation

Disease: Bleeding Disorder, Platelet-type, 15