Rabbit anti-Human, Mouse NMT2 Polyclonal Antibody | anti-NMT2 antibody

Anti-NMT2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102073

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: NMT2; Polyclonal Antibody; Anti-NMT2 Antibody; Rabbit Polyclonal; NMT2 Antibody; Rabbit PAb; Antigen Affinity Purified; N-myristoyltransferase 2; anti-NMT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NMT2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NMT2 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Human NMT2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human NMT2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining NMT2 in human liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining NMT2 in human breast carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining NMT2 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-NMT2 antibody

In higher eukaryotes, two isoforms of NMT have been identified (NMT1 and NMT2) which share about 76% amino acid sequence identity in humans. NMT2 (N-Myristoyltransferase 2) is a Protein Coding gene. The NMT2 gene, located on 10p13, is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, and frog. This gene encodes one of two N-myristoyltransferase proteins. N-terminal myristoylation is a lipid modification that is involved in regulating the function and localization of signaling proteins. The encoded protein catalyzes the addition of a myristoyl group to the N-terminal glycine residue of many signaling proteins, including the human immunodeficiency virus type 1 (HIV-1) proteins, Gag and Nef. Diseases associated with NMT2 include Human Immunodeficiency Virus Type 1. Among its related pathways are Signaling by GPCR and Metabolism of fat-soluble vitamins.

References

Sharma RK, et al. (2014) Regulation of n-myristoyltransferase by the calpain and caspase systems. Indian J Biochem Biophys 51 (6): 506-511.Selvakumar P, et al. (2009) Biochemical characterization of bovine brain myristoyl-coa:Protein n-myristoyltransferase type 2. J Biomed Biotechnol 2009 907614.Bong JJ, et al. (2009) Comparison of gene expression profiling between bovine subcutaneous and intramuscular adipose tissues by serial analysis of gene expression. Cell Biol Int 34 (1): 125-133.

NCBI and Uniprot Product Information

• NCBI GI #: 4758816
• NCBI GeneID: 9397
• NCBI Accession #: NP_004799.1
• NCBI GenBank Nucleotide #: NM_004808.2
• UniProt Accession #: O60551; Q53Y38; Q5VUC8; Q9BRB4; B0YJ49
• Molecular Weight: 56,980 Da
• NCBI Official Full Name: glycylpeptide N-tetradecanoyltransferase 2
• NCBI Official Synonym Full Names: N-myristoyltransferase 2
• NCBI Official Symbol: NMT2
• NCBI Protein Information: glycylpeptide N-tetradecanoyltransferase 2; NMT 2; glycylpeptide N-tetradecanoyltransferase 2 variant 3; myristoyl-CoA:protein N-myristoyltransferase 2; peptide N-myristoyltransferase 2; type II N-myristoyltransferase
• UniProt Protein Name: Glycylpeptide N-tetradecanoyltransferase 2
• Protein Family: Glycylpeptide N-tetradecanoyltransferase
• UniProt Gene Name: NMT2
• UniProt Synonym Gene Names: NMT 2
• UniProt Entry Name: NMT2_HUMAN

NCBI Description

N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The predicted 498-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases. [provided by RefSeq, Jul 2008]

Uniprot Description

NMT2: Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Belongs to the NMT family.

Protein type: EC 2.3.1.97; Transferase

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: Golgi apparatus; extrinsic to membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: glycylpeptide N-tetradecanoyltransferase activity

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; regulation of rhodopsin mediated signaling; viral reproduction; viral protein processing; N-terminal peptidyl-glycine N-myristoylation; virus assembly