Rabbit SREBF2 Polyclonal Antibody | anti-SREBF2 antibody

Anti-SREBF2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101779

• Gene Names: SREBF2; SREBP2; bHLHd2; SREBP-2
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: SREBF2; Polyclonal Antibody; Anti-SREBF2 Antibody; Rabbit Polyclonal; SREBF2 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-bHLHd2 Antibody; Anti-SREBP-2 Antibody; Anti-SREBP2 Antibody; Anti-SRPRA Antibody; anti-SREBF2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SREBF2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-SREBF2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human SREBF2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human SREBF2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SREBF2 rabbit polyclonal antibody at 1:500 dilutionLane A: MOLT-4 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:124 kDaObserved band size:124 kDa)

Related Product Information for anti-SREBF2 antibody

The sterol regulatory element-binding factor-2 (SREBF2) gene is a bifunctional locus encoding SREBP-2, a well-known transcriptional regulator of genes involved in cholesterol biosynthesis, and microRNA-33a. The SREBF2 gene encodes a transcription factor that activates the expression of many genes involved in the synthesis and uptake of cholesterol, fatty acids, triglycerides, and phospholipids. SREBF2 spans 72 kb and is composed of 19 exons and 18 introns. The locations of the exon/intron boundaries of SREBF2 are remarkably similar to those of SREBF1, but SREBF2 is approximately 2.8 times larger in size. A perfect 10-bp sterol regulatory element (SRE)-1 sequence was present in the promoter region of SREBF2. No SRE-1 was identified in the 5'-flanking sequences of either SREBF1a or SREBF1c, but several E-box sequences were present in SREBP1c. The SREBF2 gene plays an important role in regulating lipid homeostasis.

References

Shao F, et al. (2014) Expression of mir-33 from an srebf2 intron targets the fto gene in the chicken. PLoS One 9 (3): e91236.Miserez AR, et al. (1997) Structure of the human gene encoding sterol regulatory element binding protein 2 (srebf2). Genomics 40 (1): 31-40.Bommer GT, et al. (2011) Regulation of lipid homeostasis by the bifunctional srebf2-mir33a locus. Cell Metab 13 (3): 241-247.

NCBI and Uniprot Product Information

• NCBI GI #: 451330
• NCBI GeneID: 6721
• UniProt Accession #: Q12772; Q05BD5; Q6GTH7; Q86V36; Q9UH04
• Molecular Weight: 123,688 Da
• NCBI Official Full Name: sterol regulatory element binding protein-2
• NCBI Official Synonym Full Names: sterol regulatory element binding transcription factor 2
• NCBI Official Symbol: SREBF2
• NCBI Official Synonym Symbols: SREBP2; bHLHd2; SREBP-2
• NCBI Protein Information: sterol regulatory element-binding protein 2; class D basic helix-loop-helix protein 2; sterol regulatory element-binding transcription factor 2
• UniProt Protein Name: Sterol regulatory element-binding protein 2
• Protein Family: Sterol regulatory element-binding protein
• UniProt Gene Name: SREBF2
• UniProt Synonym Gene Names: BHLHD2; SREBP2; SREBP-2; bHLHd2
• UniProt Entry Name: SRBP2_HUMAN

NCBI Description

This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

SREBP-2: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway. Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes. Belongs to the SREBP family.

Protein type: Transcription factor; Membrane protein, multi-pass; Membrane protein, integral; DNA-binding

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; C-8 sterol isomerase activity

Biological Process: cholesterol metabolic process; transcription, DNA-dependent; response to low density lipoprotein stimulus; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; lipid metabolic process; cellular lipid metabolic process