ELISA (EIA)
(ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen rCor a 1 and allergen-specific human plasma IgG4 antibodies of Corylus avellana positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibody, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated rCor a 1.)
rCor a 1 Recombinant Protein | rCora1 recombinant protein
rCor a 1 (Corylus avellana 1.0401)
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Synonyms
rCor a 1; rCor a 1 (Corylus avellana 1.0401); rCora1 recombinant protein
Host
E Coli
Purity/Purification
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Form/Format
Purified Azide Free. Lyophilized from a 0.22um filtered solution in Tris buffer (100mM Tris, 150mM NaCl, pH8), preservative- and carrier-free. Purity >95% (HPLC method).
Applicable Applications for rCora1 recombinant protein
Flow Cytometry (FC/FACS), ELISA (EIA)
Species
Corylus avellana 1.0401
Preparation and Storage
The product is shipped at ambient temperature. Transport conditions, including short-term elevated temperature, do not impair product performance characteristics or do not affect stated product expiry date. Upon receipt, store and handle as directed.
Store at -20 degree C to -80 degree C.
Do not use after the expiry date stated on the product labels.
Reconstitute lyophilized product before use. Use reconstituted product immediately or aliquot for further storage.
Store product aliquots at -20 degree C to -80 degree C. Use within 6 months.
Avoid repeated freeze/thaw cycles.
Store at -20 degree C to -80 degree C.
Do not use after the expiry date stated on the product labels.
Reconstitute lyophilized product before use. Use reconstituted product immediately or aliquot for further storage.
Store product aliquots at -20 degree C to -80 degree C. Use within 6 months.
Avoid repeated freeze/thaw cycles.
ELISA (EIA)
(ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen rCor a 1 and allergen-specific human plasma IgG4 antibodies of Corylus avellana positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibody, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated rCor a 1.)
ELISA (EIA)
(ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen rCor a 1 and allergen-specific human plasma IgG4 antibodies of Corylus avellana positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibody, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated rCor a 1.)
Testing Data
(Recombinant allergen rCor a 1 purity verification. 5 ug of rCor a 1 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.)
Testing Data
(Recombinant allergen rCor a 1 purity verification. 5 ug of rCor a 1 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.)
Flow Cytometry (FC/FACS)
(Flow cytometry dot-plot staining pattern of rCor a 1 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies of BasoFlowEx kit.)
Flow Cytometry (FC/FACS)
(Flow cytometry dot-plot staining pattern of rCor a 1 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies of BasoFlowEx kit.)
Related Product Information for rCora1 recombinant protein
Recombinant protein rCor a 1 is expressed in Escherichia coli. DNA sequence encoding 174 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,1kDa.
NCBI and Uniprot Product Information
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Product Notes
The rCora1 cora1.0401 (Catalog #AAA1801753) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's rCor a 1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the rCora1 cora1.0401 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "rCor a 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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