Mouse anti-Human JAM-A/Junctional Adhesion Molecule Monoclonal Antibody | anti-F11R antibody

Anti-JAM-A/Junctional Adhesion Molecule A Antibody (APC), Mouse Monoclonal

Cat. Num. AAA8101626

• Gene Names: F11R; JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: JAM-A/Junctional Adhesion Molecule; Monoclonal Antibody; Anti-JAM-A/Junctional Adhesion Molecule A Antibody (APC); Mouse Monoclonal; JAM-A/F11R Antibody (APC); Mouse MAb; F11 receptor; Anti-CD321 Antibody; Anti-JAM Antibody; Anti-JAM1 Antibody; Anti-JAMA Antibody; Anti-JCAM Antibody; Anti-KAT Antibody; Anti-PAM-1 Antibody; anti-F11R antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 04
• Specificity: Human JAM-A/F11R/CD321
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 2ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-F11R antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human JAM-A/F11R/CD321 protein
• Conjugation: APC
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human JAM-A/F11R/CD321 (rh JAM-A/F11R/CD321) and conjugated with APC under optimum conditions, the unreacted APC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Testing Data

(Profile of anti-JAM-A (CD321) reactivity on SKBR3 cells analyzed by flow cytometry.)

Related Product Information for anti-F11R antibody

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.

References

Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.

NCBI and Uniprot Product Information

• NCBI GI #: 8393638
• NCBI GeneID: 50848
• NCBI Accession #: NP_058642.1
• NCBI GenBank Nucleotide #: NM_016946.4
• UniProt Accession #: Q9Y624
• Molecular Weight: 32,583 Da
• NCBI Official Full Name: junctional adhesion molecule A
• NCBI Official Synonym Full Names: F11 receptor
• NCBI Official Symbol: F11R
• NCBI Official Synonym Symbols: JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1
• NCBI Protein Information: junctional adhesion molecule A; platelet F11 receptor; platelet adhesion molecule 1; junctional adhesion molecule 1
• UniProt Protein Name: Junctional adhesion molecule A
• Protein Family: Junctional adhesion molecule
• UniProt Gene Name: F11R
• UniProt Synonym Gene Names: JAM1; JCAM; JAM-A; JAM-1; PAM-1
• UniProt Entry Name: JAM1_HUMAN

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]

Uniprot Description

JAM-A: Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. Belongs to the immunoglobulin superfamily.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1q21.2-q21.3

Cellular Component: microtubule cytoskeleton; tight junction; integral to membrane; plasma membrane; intercellular junction; cytoplasmic vesicle; cell junction

Molecular Function: protein binding; PDZ domain binding

Biological Process: intercellular junction assembly and maintenance; response to radiation; extracellular matrix organization and biogenesis; viral reproduction; transforming growth factor beta receptor signaling pathway; actomyosin structure organization and biogenesis; inflammatory response; cell adhesion; blood coagulation; leukocyte migration; positive regulation of blood pressure; intestinal absorption