Immunofluorescence (IF)
(Immunofluorescence staining of JMJD6 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human JMJD6 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus..)
Rabbit JMJD6 Polyclonal Antibody | anti-JMJD6 antibody
Anti-JMJD6 Antibody, Rabbit Polyclonal
Gene Names
JMJD6; PSR; PTDSR; PTDSR1
Reactivity
HumanPredicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
JMJD6; Polyclonal Antibody; Anti-JMJD6 Antibody; Rabbit Polyclonal; JMJD6 Antibody; Rabbit PAb; Antigen Affinity Purified; jumonji domain containing 6; Anti-PSR Antibody; Anti-PTDSR Antibody; Anti-PTDSR1 Antibody; anti-JMJD6 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human JMJD6
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-JMJD6 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
ICC/IF: 1:1500-1:50,000
ICC/IF: 1:1500-1:50,000
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human JMJD6
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human JMJD6, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of JMJD6 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human JMJD6 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus..)
Immunofluorescence (IF)
(Immunofluorescence staining of JMJD6 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human JMJD6 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus..)
Western Blot (WB)
(Anti-JMJD6 rabbit polyclonal antibody at 1:500 dilutionLane A: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:50 kDa)
Western Blot (WB)
(Anti-JMJD6 rabbit polyclonal antibody at 1:500 dilutionLane A: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:50 kDa)
Related Product Information for anti-JMJD6 antibody
JMJD6 (Jumonji Domain Containing 6, Arginine Demethylase And Lysine Hydroxylase) is a Protein Coding gene. This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. JMJD6 is a member of the Jumonji C family of Fe(II) and 2-oxoglutarate (2OG) dependent oxygenases. It possesses unique bi-functional oxygenase activities, acting as both an arginine demethylase and a lysyl-hydroxylase. JMJD6 has been reported to be over-expressed in oral, breast, lung, and colon cancers and plays important roles in the regulation of transcription through interactions with transcription regulator BRD4, histones, U2AF65, Luc7L3, and SRSF11. Diseases associated with JMJD6 include Myasthenic Syndrome, Congenital, 9, Associated With Acetylcholine Receptor Deficiency and Pancreatitis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
403
NCBI Official Full Name
bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 isoform 1
NCBI Official Synonym Full Names
jumonji domain containing 6
NCBI Official Symbol
JMJD6
NCBI Official Synonym Symbols
PSR; PTDSR; PTDSR1
NCBI Protein Information
bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; phosphatidylserine receptor; jmjC domain-containing protein 6; histone arginine demethylase JMJD6; peptide-lysine 5-dioxygenase JMJD6; jumonji domain-containing protein 6
UniProt Protein Name
Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
Protein Family
UniProt Gene Name
JMJD6
UniProt Synonym Gene Names
KIAA0585; PTDSR; Protein PTDSR
UniProt Entry Name
JMJD6_HUMAN
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Product Notes
The JMJD6 jmjd6 (Catalog #AAA8100610) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-JMJD6 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:1000 ICC/IF: 1:1500-1:50,000. Researchers should empirically determine the suitability of the JMJD6 jmjd6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMJD6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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