Rabbit JMJD6 Polyclonal Antibody | anti-JMJD6 antibody

JMJD6 Antibody

Cat. Num. AAA9414575

• Gene Names: JMJD6; PSR; PTDSR; PTDSR1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: JMJD6; Polyclonal Antibody; JMJD6 Antibody; Jumonji domain-containing protein 6; phosphatidylserine receptor; PTDSR; PSR; anti-JMJD6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 414

• Applicable Applications for anti-JMJD6 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 15 amino acid peptide from near the amino terminus of human JMJD6.
• Target Name: JMJD6
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of JMJD6 in human brain tissue lysate with JMJD6 antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of JMJD6 in rat brain tissue with JMJD6 antibody at 2.5 ug/mL.)

Related Product Information for anti-JMJD6 antibody

The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. JMJD6 was initially identified as a membrane protein that participates in phagocytosis but recent studies have shown that it has other functions when it is expressed in the cytosol and nucleus. JMJD6 is thought to play important roles in regulation of development and differentiation as knockdown experiments in mice resulted in neonatal lethality with severe defects in the morphology of numerous organs. JMJD6 also can catalyze the lysyl-hydroxylation of U2AF65, a protein involved with RNA splicing, suggesting that some of the functions attributed to JMJD6 may be due to its regulatory activity of RNA splicing.

Product Categories/Family for anti-JMJD6 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 125988395
• NCBI GeneID: 23210
• NCBI Accession #: NP_001074930.1
• NCBI GenBank Nucleotide #: NM_001081461.1
• UniProt Accession #: Q6NYC1; Q86VY0; Q8IUM5; Q9Y4E2; B3KMN8; B4DGX1
• Molecular Weight: 47,626 Da
• NCBI Official Full Name: bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 isoform 1
• NCBI Official Synonym Full Names: jumonji domain containing 6
• NCBI Official Symbol: JMJD6
• NCBI Official Synonym Symbols: PSR; PTDSR; PTDSR1
• NCBI Protein Information: bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
• UniProt Protein Name: Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
• Protein Family: Bifunctional arginine demethylase and lysyl-hydroxylase
• UniProt Gene Name: JMJD6
• UniProt Synonym Gene Names: KIAA0585; PTDSR; Protein PTDSR
• UniProt Entry Name: JMJD6_HUMAN

NCBI Description

This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

JMJD6: Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses. Belongs to the JMJD6 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-; Receptor, misc.; Apoptosis; Nucleolus; Oxidoreductase; Cell development/differentiation

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleoplasm; plasma membrane; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; histone demethylase activity (H4-R3 specific); single-stranded RNA binding; RNA binding; iron ion binding; receptor activity; histone demethylase activity (H3-R2 specific)

Biological Process: erythrocyte development; transcription, DNA-dependent; heart development; RNA splicing; T cell differentiation in the thymus; recognition of apoptotic cell; peptidyl-lysine hydroxylation to 5-hydroxy-L-lysine; regulation of nuclear mRNA splicing, via spliceosome; macrophage activation; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; retina development in camera-type eye; sprouting angiogenesis; kidney development; mRNA processing; lung development