Rabbit anti-Human Pumilio 2/PUM2 Polyclonal Antibody | anti-PUM2 antibody

Anti-Pumilio 2/PUM2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100553

• Gene Names: PUM2; PUMH2; PUML2
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Pumilio 2/PUM2; Polyclonal Antibody; Anti-Pumilio 2/PUM2 Antibody; Rabbit Polyclonal; PUM2 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-PUMH2 Antibody; Anti-PUML2 Antibody; anti-PUM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PUM2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PUM2 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human PUM2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human PUM2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human PUM2 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-PUM2 antibody

PUM2 is a member of the highly conserved Puf family of RNA binding proteins that often function as gene-specific translation regulators. PUM2 pull-down methods combined with reverse transcription and RT-PCR confirmed that PUM2 does indeed bind P2P-R mRNA. These results suggest that P2P-R expression may be translationally regulated by PUM2 and that P2P-R may modulate translation by influencing ribosomal protein gene expression. The human genome contains two Pumilio related genes, PUM1 and PUM2, that encode 127 and 114 kDa proteins with evolutionarily highly conserved Pum RNA-binding domain (86 and 88% homology with the fly Pum protein). PUM1 and PUM2 proteins share 83% overall similarity, with RNA-binding domain being 91% identical. PUM1 consists of 22 exons, spanning about 150 kb on chromosome 1p35.2, whereas PUM2 consists of 20 exons and spans at least 80 kb on chromosome 2p23-24.

NCBI and Uniprot Product Information

• NCBI GI #: 544583547
• NCBI GeneID: 23369
• NCBI Accession #: NP_001269681.1
• NCBI GenBank Nucleotide #: NM_001282752.1
• UniProt Accession #: Q8TB72; O00234; Q53TV7; Q8WY43; Q9HAN2; B3KSL0; B4E2B6; D6W527
• Molecular Weight: 107,646 Da
• NCBI Official Full Name: pumilio homolog 2 isoform 2
• NCBI Official Synonym Full Names: pumilio RNA-binding family member 2
• NCBI Official Symbol: PUM2
• NCBI Official Synonym Symbols: PUMH2; PUML2
• NCBI Protein Information: pumilio homolog 2; pumilio-2
• UniProt Protein Name: Pumilio homolog 2
• Protein Family: Pumilio
• UniProt Gene Name: PUM2
• UniProt Synonym Gene Names: KIAA0235; PUMH2; Pumilio-2
• UniProt Entry Name: PUM2_HUMAN

NCBI Description

This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

Pumilio-2: Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Motility/polarity/chemotaxis; Translation

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: nuclear membrane; stress granule; perinuclear region of cytoplasm; cytoplasm

Molecular Function: protein binding

Biological Process: regulation of translation; stress granule assembly