Rabbit anti-Human; Rat WDR45 Polyclonal Antibody | anti-WDR45 antibody

WDR45 Antibody: ATTO 390

Cat. Num. AAA809797

• Gene Names: WDR45; JM5; NBIA4; NBIA5; WDRX1; WIPI4; WIPI-4
• Reactivity: Human; Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Peptide Affinity Purified
• Synonyms: WDR45; Polyclonal Antibody; WDR45 Antibody: ATTO 390; Rabbit Anti-Human WDR45 Polyclonal; WIPI4 Antibody; WDR45 Antibody; WDRX1 Antibody; WD repeat domain phosphoinositide-interacting protein 4 Antibody; WIPI-4 Antibody; WDRXI4 Antibody; WD repeat-containing protein 45 Antibody; JM5 Antibody; anti-WDR45 antibody

• Host: Rabbit
• Reactivity: Human; Rat
• Clonality: Polyclonal
• Specificity: Detects ~40 kDa.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 360

• Applicable Applications for anti-WDR45 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000; ICC/IF: 1:100
• Target: WDR45
• Immunogen: Synthetic peptide from the C-terminal of Human WIPI4 (aa. 312-322)
• Immunogen Species: Human
• Conjugate: ATTO 390
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Tissue Specificity: Ubiquitously expressed, with high expression in skeletal muscle and heart. Weakly expressed in liver and placenta. Expression is down-regulated in pancreatic and in kidney tumors.
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 40 kDa WDR45 protein using Rabbit Anti-WDR45 Polyclonal Antibody (SPC-651). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody (SPC-651) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 40 kDa.)

Related Product Information for anti-WDR45 antibody

WIPI-4 is a 360 amino acid protein containing three WD repeats. Existing as three alternatively spliced isoforms, WIPI-4 is ubiquitously expressed but found at highest levels in heart and skeletal muscle. WD repeat proteins are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Diseases associated with this gene include neurodegeneration with brain iron accululation 5 and 4.

Product Categories/Family for anti-WDR45 antibody

Antibodies; Cancer; Autophagy; Cell Signaling; Apoptosis

References

1. http://www.genecards.org/cgi-bin/carddisp.pl?gene=WDR45 2. Strausberg R.L., et al. (2002) Proc Natl Acad Sci U S A. 99(26): 16899-16903. 3. Haack T.B., et al. (2012) Am J Hum Genet. 91(6): 1144-1149.

NCBI and Uniprot Product Information

• NCBI GI #: 71483646
• NCBI GeneID: 11152
• NCBI Accession #: NP_001025067.1
• NCBI GenBank Nucleotide #: NM_001029896.1
• UniProt Accession #: Q9Y484; Q5MNZ5; Q6IBS7; Q6NT94; Q96H03; A6NGH5; B7WPI2
• Molecular Weight: 39,939 Da
• NCBI Official Full Name: WD repeat domain phosphoinositide-interacting protein 4 isoform 2
• NCBI Official Synonym Full Names: WD repeat domain 45
• NCBI Official Symbol: WDR45
• NCBI Official Synonym Symbols: JM5; NBIA4; NBIA5; WDRX1; WIPI4; WIPI-4
• NCBI Protein Information: WD repeat domain phosphoinositide-interacting protein 4
• UniProt Protein Name: WD repeat domain phosphoinositide-interacting protein 4
• Protein Family: WD repeat domain phosphoinositide-interacting protein
• UniProt Gene Name: WDR45
• UniProt Synonym Gene Names: WDRX1; WDRXI4; WIPI4; WIPI-4

NCBI Description

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an important role in the autophagy pathway, which is the major intracellular degradation system by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation.