Rabbit ULK3 Polyclonal Antibody | anti-ULK3 antibody

ULK3 Antibody: ATTO 594

Cat. Num. AAA809728

• Reactivity: Human; Mouse; Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Peptide Affinity Purified
• Synonyms: ULK3; Polyclonal Antibody; ULK3 Antibody: ATTO 594; Rabbit Anti-Human ULK3 Polyclonal; Serine/threonine-protein kinase ULK3 Antibody; EC:2.7.11.1 Antibody; Ulk3 Antibody; DKFZP434C131 Antibody; Unc-51-like kinase 3 Antibody; FLJ90566 Antibody; ULK3_HUMAN Antibody; unc 51 like kinase 3 (C. elegans) Antibody; ULK3 Antibody; anti-ULK3 antibody

• Host: Rabbit
• Reactivity: Human; Mouse; Rat
• Clonality: Polyclonal
• Specificity: Detects ~53 kDa.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 472

• Applicable Applications for anti-ULK3 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000; ICC/IF: 1:100
• Target: ULK3
• Immunogen: Synthetic peptide from the N-terminal of Human ULK3 (aa. 166-177)
• Immunogen Species: Human
• Cellular Localization: Cytoplasm
• Conjugate: ATTO 594
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Tissue Specificity: Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord.
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 53.4 kDa ULK3 protein using Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 53.4 kDa. Other Band(s): ~25 kDa in all lysates.)

Western Blot (WB)

(Western blot analysis of Rat Brain cell lysates showing detection of 53.4 kDa ULK3 protein using Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 53.4 kDa.)

Related Product Information for anti-ULK3 antibody

UNC-51 like kinase 3, ULK3, is a serine-threonine protein kinase that acts as a regulator of SHH signaling and autophagy. Is phosphorylates in vitro GLI2.

Product Categories/Family for anti-ULK3 antibody

Antibodies; Cell Signaling; Protein Phosphorylation; Serine/Threonine Kinases

References

1. Malovergan A., et al. (2010) J Biol Chem. 285(39): 30079-30090. 2. Young A.R., et al. (2009) Genes Dev. 23(7):798-803.

NCBI and Uniprot Product Information

• NCBI GI #: 656214624
• NCBI GeneID: 25989
• NCBI Accession #: NP_001092906.3
• NCBI GenBank Nucleotide #: NM_001099436.3
• UniProt Accession #: Q6PHR2; Q9NPN5; Q9UFS4; B2RXK3; B4DFT0; B4DRQ7; D3DW68
• Molecular Weight: 54,997 Da
• NCBI Official Full Name: serine/threonine-protein kinase ULK3 isoform a
• NCBI Official Synonym Full Names: unc-51 like kinase 3
• NCBI Official Symbol: ULK3
• NCBI Protein Information: serine/threonine-protein kinase ULK3
• UniProt Protein Name: Serine/threonine-protein kinase ULK3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: ULK3

Uniprot Description

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.