ULK3 Polyclonal Antibody | anti-ULK3 antibody

Anti-ULK3 Antibody

Cat. Num. AAA177781

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: ULK3; Polyclonal Antibody; Anti-ULK3 Antibody; Serine/threonine-protein kinase ULK3; DKFZP434C131; FLJ90566; Ulk3; ULK3_HUMAN; unc 51 like kinase 3 (C. elegans); Unc-51-like kinase 3; unc-51 like kinase 3; anti-ULK3 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 472

• Applicable Applications for anti-ULK3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at C-terminus of human ULK3 (427-454aa HTEVQNLMARAEYLKEQVKMRESRWEAD), different from the related mouse and rat sequences by four amino acids.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- ULK3 Picoband antibody, MBS177781, Western blottingAll lanes: Anti ULK3 (MBS177781) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Testis Tissue Lysate at 50ugLane 3: Mouse Brain Tissue Lysate at 50ugLane 4: Human Placenta Tissue Lysate at 50ugLane 5: 22RV1 Whole Cell Lysate at 40ugLane 6: SMMC Whole Cell Lysate at 40ugPredicted bind size: 53KDObserved bind size: 53KD)

Immunohistochemistry (IHC)

(Anti- ULK3 Picoband antibody, MBS177781, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti- ULK3 Picoband antibody, MBS177781, IHC(P)IHC(P): Human Lung Cancer Tissue )

Related Product Information for anti-ULK3 antibody

Description: Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase ULK3(ULK3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. In search of potential homologues to Drosophila and human Fu, people have cloned human serine/threonine kinase ULK3 and assessed its ability to regulate GLI transcription factors, mediators of SHH signaling. And ULK3 enhances endogenous and over-expressed GLI1 and GLI2 transcriptional activity in cultured cells, as assessed by GLI-luciferase reporter assay. Besides that, ULK3 alters subcellular localization of GLI1, as assessed by immunofluorescent staining and immunoblotting assays. It is showed that ULK3 is an autophosphorylated kinase and phosphorylates GLI proteins in vitro. Also, ULK3 catalytical activity is crucial for its function in SHH pathway. It is demonstrated that ULK3 is widely expressed and its expression is higher in a number of tissues where Shh signaling is known to be active. The data suggest that serine/threonine kinase ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.

References

1. Maloverjan A; Piirsoo M; Michelson P; Kogerman P; Osterlund T. "Identification of a novel serine/threonine kinase ULK3 as a positive regulator of Hedgehog pathway". Exp Cell Res, 2010 Feb 15. 

NCBI and Uniprot Product Information

• NCBI GI #: 656214624
• NCBI GeneID: 25989
• NCBI Accession #: NP_001092906.3
• NCBI GenBank Nucleotide #: NM_001099436.3
• UniProt Accession #: Q6PHR2; Q9NPN5; Q9UFS4; B2RXK3; B4DFT0; B4DRQ7; D3DW68
• Molecular Weight: 54,997 Da
• NCBI Official Full Name: serine/threonine-protein kinase ULK3 isoform a
• NCBI Official Synonym Full Names: unc-51 like kinase 3
• NCBI Official Symbol: ULK3
• NCBI Protein Information: serine/threonine-protein kinase ULK3
• UniProt Protein Name: Serine/threonine-protein kinase ULK3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: ULK3
• UniProt Entry Name: ULK3_HUMAN

Uniprot Description

ULK3: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; EC 2.7.11.1; Other group; ULK family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: cytoplasm

Molecular Function: ATP binding; protein binding; protein serine/threonine kinase activity

Biological Process: autophagy; negative regulation of smoothened signaling pathway; positive regulation of smoothened signaling pathway; protein amino acid autophosphorylation