Mouse 7-Ketocholesterol Monoclonal Antibody | anti-7-KC antibody

7-Ketocholesterol Antibody, Clone 3F7: FITC

Cat. Num. AAA808748

• Reactivity: Species Independent
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, ELISA
• Purity: Protein G Purified
• Synonyms: 7-Ketocholesterol; Monoclonal Antibody; 7-Ketocholesterol Antibody; Clone 3F7: FITC; Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG3; 7-Ketocholesterol Antibody: FITC; 7-Ketocholesterol [7-KC] Antibody; 7-KC Antibody; 7-ketocholesterol (7-KC) Antibody; 7KC Antibody; 7KC-Modified Keyhole-Lympet Hemocyanine Antibody; 7KC-Modified KLH Antibody; anti-7-KC antibody

• Host: Mouse
• Reactivity: Species Independent
• Clonality: Monoclonal
• Isotype: IgG3
• Clone Number: 3F7
• Specificity: Specific for 7-ketocholesterol modified proteins.
• Purity/Purification: Protein G Purified
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-7-KC antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:1000; ICC/IF: 1:50; ELISA: 1:1000
• Target: 7-Ketocholesterol
• Immunogen: Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
• Conjugate: FITC
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Preparation and Storage: Store at -20 degree C.

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Western Blot (WB)

(Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol protein using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (2.0 ug). Lane 4: 7-ketocholesterol-BSA (0.5 ug). Lane 5: 7-ketocholesterol-BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)

Product Categories/Family for anti-7-KC antibody

Antibodies; Cancer; Oxidative Stress; Lipid peroxidation