Rabbit Aquaporin 4 Polyclonal Antibody | anti-Aqp4 antibody

Aquaporin 4 Antibody: RPE

Cat. Num. AAA806737

• Gene Names: Aqp4; Miwc; AQP-4; MIWC2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Synonyms: Aquaporin 4; Polyclonal Antibody; Aquaporin 4 Antibody: RPE; AQP4; anti-Aqp4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Affinity Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 323

• Applicable Applications for anti-Aqp4 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: 1:1000-4000 (WB), 1:400 (IF)
• Conjugate: RPE
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 um cryostat sections.)

Western Blot (WB)

(Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 4 protein using Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505). Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505) at 1:2000.)

Related Product Information for anti-Aqp4 antibody

Background Info: Detects ~35kDa. May detect larger glycosylated bands ~50kDa.

Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 4 is constitutively expressed in the basolateral cell membrane of principal collecting duct cells in the kidney and provide a pathway for water to exit these cells (3). It is also expressed in astrocytes and is up regulated by direct insult to the CNS (4). Aquaporin 4 has been proposed as the primary autoimmune target in neuromyelitis optica (5, 6).

Product Categories/Family for anti-Aqp4 antibody

Transporters

References

1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. Agre P., Nielsen S. (1996) Nephrologie. 17(7): 409-415. 4. Nagelhus E.A., Mathiisen T.M., Ottersen O.P. (2004) Neuroscience. 129(4): 905-913. 5. Lennon V.A., Kryzer T.J., Pittock S.J., Verkman A.S., Hinson S.R. (2005) J Exp Med. 202(4): 473- 477. 6. Bradl M., Lassmann D.H. (2008) Int. MS Journal/ MS Forum. 15(3): 75-78.

NCBI and Uniprot Product Information

• NCBI GI #: 6978531
• NCBI GeneID: 25293
• NCBI Accession #: NP_036957.1
• NCBI GenBank Nucleotide #: NM_012825.4
• UniProt Accession #: P47863
• Molecular Weight: 28,768 Da
• NCBI Official Full Name: aquaporin-4 isoform 1
• NCBI Official Synonym Full Names: aquaporin 4
• NCBI Official Symbol: Aqp4
• NCBI Official Synonym Symbols: Miwc; AQP-4; MIWC2
• NCBI Protein Information: aquaporin-4; WCH4; mercurial-insensitive water channel
• UniProt Protein Name: Aquaporin-4
• Protein Family: Aquaporin
• UniProt Gene Name: Aqp4
• UniProt Synonym Gene Names: AQP-4; MIWC
• UniProt Entry Name: AQP4_RAT

NCBI Description

The protein encoded by this gene forms a water-specific channel that regulates body water balance, particularly in the brain. The encoded protein acts as a membrane-bound homotetramer, interacting with other proteins (MLC1, TRPV4, HEPACAM, and ATP1B1) to form a complex. Several variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described

Protein type: Transporter, aquaporin family; Transporter; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: protein complex; membrane; cell projection membrane; integral to plasma membrane; basolateral plasma membrane; T-tubule; cytoplasm; plasma membrane; basal plasma membrane; intercellular junction; sarcolemma; external side of plasma membrane

Molecular Function: water channel activity; porin activity; glycerol channel activity

Biological Process: water homeostasis; glycerol transport; response to radiation; cellular water homeostasis; sensory perception of sound; hyperosmotic salinity response; water transport; carbon dioxide transport; response to glucocorticoid stimulus; negative regulation of interleukin-6 production; multicellular organismal water homeostasis; negative regulation of interleukin-1 beta production; female pregnancy; transmembrane transport; protein homooligomerization