Rabbit NCC Polyclonal Antibody | anti-SLC12A3 antibody

NCC Antibody: ATTO 390

Cat. Num. AAA805495

• Gene Names: SLC12A3; NCC; TSC; NCCT
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Synonyms: NCC; Polyclonal Antibody; NCC Antibody: ATTO 390; Slc12a3; anti-SLC12A3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Affinity Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1030

• Applicable Applications for anti-SLC12A3 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: 1:1000 (WB)
• Conjugate: ATTO 390
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).)

Western Blot (WB)

(Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:1000.)

Related Product Information for anti-SLC12A3 antibody

Background Info: Detects ~160kDa

Scientific Background: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).

Product Categories/Family for anti-SLC12A3 antibody

Ion Channels; Transporters

References

1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.

NCBI and Uniprot Product Information

• NCBI GI #: 186910315
• NCBI GeneID: 6559
• NCBI Accession #: NP_000330; NP_062218
• NCBI GenBank Nucleotide #: NM_000339.2
• UniProt Accession #: P55018; P55017
• Molecular Weight: 110,554 Da
• NCBI Official Full Name: solute carrier family 12 member 3 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 12 (sodium/chloride transporter), member 3
• NCBI Official Symbol: SLC12A3
• NCBI Official Synonym Symbols: NCC; TSC; NCCT
• NCBI Protein Information: solute carrier family 12 member 3; Na-Cl symporter; Na-Cl cotransporter; thiazide-sensitive Na-Cl cotransporter; thiazide-sensitive sodium-chloride cotransporter; NaCl electroneutral thiazide-sensitive cotransporter
• UniProt Protein Name: Solute carrier family 12 member 3
• Protein Family: Solute carrier family
• UniProt Gene Name: Slc12a3
• UniProt Synonym Gene Names: Tsc
• UniProt Entry Name: S12A3_RAT

NCBI Description

This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NCCT: Key mediator of sodium and chloride reabsorption in this nephron segment, accounting for a significant fraction of renal sodium reabsorption. Interacts with KLHL3. Predominant in kidney. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Transporter; Membrane protein, integral

Cellular Component: apical plasma membrane; integral to membrane; plasma membrane; cytosol; vesicle

Molecular Function: sodium ion transmembrane transporter activity; transporter activity; sodium:chloride symporter activity; cation:chloride symporter activity

Biological Process: transport; sodium ion transport; chloride transport