Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human heart slide using MBS767104 (beta actin Antibody) at dilution of 1:50)
Mouse beta actin Monoclonal Antibody
beta actin Mouse Monoclonal
Reactivity
Human, Mouse, Rat; other species are not tested. Please decide the specific by homology
Applications
ELISA, Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
Purity
Purification: Protein A+G purificationPurity: > = 95% as determined by SDS-PAGE
Synonyms
beta actin; Monoclonal Antibody; beta actin Mouse Monoclonal; ACTB; Actin; actin; beta; cytoplasmic 1; B actin; Beta actin; F actin; anti-beta actin antibody
Host
Mouse
Reactivity
Human, Mouse, Rat; other species are not tested. Please decide the specific by homology
Clonality
Monoclonal
Isotype
IgM
Purity/Purification
Purification: Protein A+G purification
Purity: > = 95% as determined by SDS-PAGE
Purity: > = 95% as determined by SDS-PAGE
Form/Format
Liquid
Applicable Applications for anti-beta actin antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS),
Application Notes
WB:1:2000-1:20000
IHC:1:50-1:500
IF:1:20-1:200
IHC:1:50-1:500
IF:1:20-1:200
Immunogen
Actin, beta
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Preparation and Storage
Store -20°C for 24 months (Avoid repeated freeze / thaw cycles.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human heart slide using MBS767104 (beta actin Antibody) at dilution of 1:50)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human heart slide using MBS767104 (beta actin Antibody) at dilution of 1:50)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS767104 (beta actin Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red))
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS767104 (beta actin Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red))
Western Blot (WB)
(A549 cells were subjected to SDS PAGE followed by western blot with MBS767104 (ACTB antibody) at dilution of 1:2000)
Western Blot (WB)
(A549 cells were subjected to SDS PAGE followed by western blot with MBS767104 (ACTB antibody) at dilution of 1:2000)
Related Product Information for anti-beta actin antibody
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
42kDa
NCBI Official Full Name
Beta-actin
Similar Products
Product Notes
The beta actin (Catalog #AAA767104) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The beta actin Mouse Monoclonal reacts with Human, Mouse, Rat; other species are not tested. Please decide the specific by homology and may cross-react with other species as described in the data sheet. AAA Biotech's beta actin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS),. WB:1:2000-1:20000 IHC:1:50-1:500 IF:1:20-1:200. Researchers should empirically determine the suitability of the beta actin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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