Rat D(1A) dopamine receptor ELISA Kit | Drd1 elisa kit

Rat D(1A) dopamine receptor ELISA Kit

Cat. Num. AAA765552

• Gene Names: DRD1; DADR; DRD1A
• Reactivity: Rat
• Synonyms: D(1A) dopamine receptor; Rat D(1A) dopamine receptor ELISA Kit; Drd1/D1R/DRD1 (Dopamine Receptor D1)/Dopamine D1 receptor; Drd1 elisa kit

• Reactivity: Rat
• Sequence Length: 446

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 12.5-800ng/ml
• Sensitivity: 7.5ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Drd1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Drd1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Drd1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Drd1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Drd1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 118228
• NCBI GeneID: 1812
• Molecular Weight: 49,293 Da
• NCBI Official Full Name: D(1A) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D1
• NCBI Official Symbol: DRD1
• NCBI Official Synonym Symbols: DADR; DRD1A
• NCBI Protein Information: D(1A) dopamine receptor
• UniProt Protein Name: D(1A) dopamine receptor
• Protein Family: D(1A) dopamine receptor
• UniProt Gene Name: DRD1
• UniProt Entry Name: DRD1_HUMAN

NCBI Description

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DRD1: a G-protein coupled receptor.One of the five types (D1 to D5) of receptors for dopamine. The most abundant dopamine receptor in the central nervous system. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Interacts with calcyon.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: caveola; cell soma; cytosol; dendritic shaft; endoplasmic reticulum membrane; integral to plasma membrane; nerve terminal; nonmotile primary cilium; nucleus; plasma membrane

Molecular Function: angiotensin receptor binding; ATPase binding; D3 dopamine receptor binding; dopamine binding; dopamine D1 receptor-like receptor activity; dopamine receptor activity; drug binding; G-protein alpha-subunit binding; protein binding; protein complex binding; protein heterodimerization activity; protein phosphatase binding

Biological Process: adenylate cyclase activation; adult walking behavior; astrocyte development; behavioral fear response; behavioral response to cocaine; calcium-mediated signaling; cellular response to insulin stimulus; cerebral cortex GABAergic interneuron migration; conditioned taste aversion; dentate gyrus development; dopamine metabolic process; dopamine receptor, adenylate cyclase activating pathway; dopamine receptor, phospholipase C activating pathway; dopamine transport; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; generation of action potential; glucose import; grooming behavior; habituation; learning; maternal behavior; mating behavior; memory; negative regulation of cell migration; negative regulation of circadian sleep/wake cycle, sleep; negative regulation of protein kinase activity; operant conditioning; orbitofrontal cortex development; peristalsis; positive regulation of cAMP biosynthetic process; positive regulation of cell migration; positive regulation of membrane potential; positive regulation of potassium ion transport; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of synaptic transmission, glutamatergic; prepulse inhibition; protein import into nucleus; regulation of dopamine metabolic process; regulation of dopamine uptake; regulation of long-term neuronal synaptic plasticity; regulation of vasoconstriction; response to activity; response to amino acid stimulus; response to amphetamine; response to drug; response to estradiol stimulus; response to ethanol; response to food; response to morphine; response to nicotine; response to retinoic acid; sensitization; social behavior; striatum development; synaptic transmission, dopaminergic; synaptogenesis; thermoregulation; transmission of nerve impulse; vasodilation; visual learning