Monkey Dopamine Receptor D1 ELISA Kit | DRD1 elisa kit

Monkey Dopamine Receptor D1 ELISA Kit

Cat. Num. AAA754881

• Gene Names: DRD1; DADR; DRD1A
• Reactivity: Monkey
• Synonyms: Dopamine Receptor D1; Monkey Dopamine Receptor D1 ELISA Kit; DRD1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This DRD1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey DRD1. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DRD1 elisa kit

Principle of the Assay: DRD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DRD1 antibody and an DRD1-HRP conjugate. The assay sample and buffer are incubated together with DRD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DRD1 concentration since DRD1 from samples and DRD1-HRP conjugate compete for the anti-DRD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DRD1 from the sample, fewer sites are left to bind DRD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DRD1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 20269979
• NCBI GeneID: 1812
• Molecular Weight: 49,293 Da
• NCBI Official Full Name: dopamine receptor D1
• NCBI Official Synonym Full Names: dopamine receptor D1
• NCBI Official Symbol: DRD1
• NCBI Official Synonym Symbols: DADR; DRD1A
• NCBI Protein Information: D(1A) dopamine receptor; D(1A) dopamine receptor; dopamine D1 receptor
• UniProt Protein Name: D(1A) dopamine receptor
• Protein Family: D(1A) dopamine receptor
• UniProt Gene Name: DRD1
• UniProt Entry Name: DRD1_HUMAN

NCBI Description

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DRD1: a G-protein coupled receptor.One of the five types (D1 to D5) of receptors for dopamine. The most abundant dopamine receptor in the central nervous system. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Interacts with calcyon.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; nucleus

Molecular Function: protein binding; dopamine receptor activity; dopamine binding; dopamine D1 receptor-like receptor activity

Biological Process: synaptic transmission, dopaminergic; peristalsis; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); behavioral fear response; prepulse inhibition; positive regulation of potassium ion transport; generation of action potential; thermoregulation; adult walking behavior; G-protein signaling, adenylate cyclase activating pathway; positive regulation of synaptic transmission, glutamatergic; vasodilation; dopamine metabolic process; synaptogenesis; protein import into nucleus; behavioral response to cocaine; conditioned taste aversion; positive regulation of cAMP biosynthetic process; visual learning; dopamine receptor, adenylate cyclase activating pathway; grooming behavior; response to drug; dopamine transport; dentate gyrus development; striatum development; cerebral cortex GABAergic interneuron migration; maternal behavior; response to amphetamine; regulation of dopamine metabolic process; adenylate cyclase activation; mating behavior; regulation of dopamine uptake; transmission of nerve impulse; astrocyte development; memory; G-protein signaling, coupled to cyclic nucleotide second messenger; habituation; glucose import; sensitization; operant conditioning; positive regulation of release of sequestered calcium ion into cytosol; dopamine receptor, phospholipase C activating pathway; positive regulation of cell migration