Human DNA mismatch repair protein Msh2 ELISA Kit | MSH2 elisa kit

Human DNA mismatch repair protein Msh2 ELISA Kit

Cat. Num. AAA764440

• Gene Names: MSH2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1
• Reactivity: Human
• Synonyms: DNA mismatch repair protein Msh2; Human DNA mismatch repair protein Msh2 ELISA Kit; MSH2/MSH2/COCA1/FCC1/HNPCC/HNPCC1/LCFS2/MutS protein homolog 2/LCFS2/DNA mismatch repair protein Msh2/hMSH2/HNPCC1mutS (E. coli) homolog 2 (colon cancer; nonpolyposis type 1)/LCFS2/mutS homolog 2; colon cancer; nonpolyposis type 1 (E. coli)/MutS protein homolog 2; MSH2 elisa kit

• Reactivity: Human
• Sequence Length: 934

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MSH2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human MSH2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MSH2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MSH2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MSH2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557761
• NCBI GeneID: 4436
• NCBI Accession #: NP_000242.1
• NCBI GenBank Nucleotide #: NM_000251.2
• Molecular Weight: 97,323 Da
• NCBI Official Full Name: DNA mismatch repair protein Msh2 isoform 1
• NCBI Official Synonym Full Names: mutS homolog 2
• NCBI Official Symbol: MSH2
• NCBI Official Synonym Symbols: FCC1; COCA1; HNPCC; LCFS2; HNPCC1
• NCBI Protein Information: DNA mismatch repair protein Msh2
• UniProt Protein Name: DNA mismatch repair protein Msh2
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: MSH2
• UniProt Synonym Gene Names: hMSH2
• UniProt Entry Name: MSH2_HUMAN

NCBI Description

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

MSH2: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. Heterodimer consisting of MSH2-MSH6 (MutS alpha) or MSH2- MSH3 (MutS beta). Both heterodimer form a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Interacts with SLX4/BTBD12; this interaction is direct and links MutS beta to SLX4, a subunit of different structure-specific endonucleases. Interacts with SMARCAD1. Ubiquitously expressed. Belongs to the DNA mismatch repair MutS family.

Protein type: DNA-binding; Tumor suppressor

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: membrane; MutSalpha complex; MutSbeta complex; nuclear chromosome, telomeric region; nucleoplasm

Molecular Function: ADP binding; ATP binding; ATPase activity; centromeric DNA binding; dinucleotide insertion or deletion binding; dinucleotide repeat insertion binding; DNA binding; double-strand/single-strand DNA junction binding; double-stranded DNA binding; enzyme binding; four-way junction DNA binding; guanine/thymine mispair binding; loop DNA binding; magnesium ion binding; mismatched DNA binding; MutLalpha complex binding; oxidized purine DNA binding; protein binding; protein C-terminus binding; protein homodimerization activity; protein kinase binding; single guanine insertion binding; single thymine insertion binding; single-stranded DNA binding; Y-form DNA binding

Biological Process: B cell differentiation; B cell mediated immunity; cell cycle arrest; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA repair; double-strand break repair; germ cell development; in utero embryonic development; intra-S DNA damage checkpoint; isotype switching; maintenance of DNA repeat elements; male gonad development; meiotic gene conversion; mismatch repair; negative regulation of DNA recombination; negative regulation of meiotic recombination; negative regulation of neuron apoptosis; oxidative phosphorylation; positive regulation of helicase activity; postreplication repair; response to UV-B; response to X-ray; somatic hypermutation of immunoglobulin genes; somatic recombination of immunoglobulin gene segments

Disease: Lynch Syndrome I; Mismatch Repair Cancer Syndrome; Muir-torre Syndrome