Rabbit anti-Human Platelet-derived Growth Factor D Polyclonal Antibody | anti-PDGF-D antibody

Platelet-derived Growth Factor D, ID (SCDGFB, PDGF-D, Iris-expressed Growth Factor, Spinal Cord-derived Growth Factor B, SCDGF-B, Pdgfd, Iegf) (Azide free) (HRP)

Cat. Num. AAA6493947

• Gene Names: Pdgfd; Scdgfb; rSCDGF-B
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Platelet-derived Growth Factor D; Polyclonal Antibody; ID (SCDGFB; PDGF-D; Iris-expressed Growth Factor; Spinal Cord-derived Growth Factor B; SCDGF-B; Pdgfd; Iegf) (Azide free) (HRP); anti-PDGF-D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Platelet-derived Growth Factor D.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PDGF-D antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 313-341 in the central region of human Platelet-derived Growth Factor D, UniProt Accession #Q9EQT1. Species sequence homology: mouse
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysate (35ug) using MBS628717 (1:1000). A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Western Blot (WB)

(Western Blot analysis in rat kidney tissue lysate (35ug) using MBS628717 (1:1000). A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS628717, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS628717 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS628717 for immunohistochemistry.)

Product Categories/Family for anti-PDGF-D antibody

Antibodies; Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 25742601
• NCBI GeneID: 66018
• NCBI Accession #: NP_076452.1
• NCBI GenBank Nucleotide #: NM_023962.2
• UniProt Accession #: Q9EQT1
• Molecular Weight: 42,128 Da
• NCBI Official Full Name: platelet-derived growth factor D
• NCBI Official Synonym Full Names: platelet derived growth factor D
• NCBI Official Symbol: Pdgfd
• NCBI Official Synonym Symbols: Scdgfb; rSCDGF-B
• NCBI Protein Information: platelet-derived growth factor D
• UniProt Protein Name: Platelet-derived growth factor D
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: Pdgfd
• UniProt Synonym Gene Names: Iegf; Scdgfb; PDGF-D; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form

NCBI Description

a growth factor with mitogenic activity in the central nervous system [RGD, Feb 2006]

Uniprot Description

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis (). May play an important role in control of lens epithelial cell proliferation. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix.