Rat Interleukin-23 subunit alpha ELISA Kit | Il23a elisa kit

Rat Interleukin-23 subunit alpha ELISA Kit

Cat. Num. AAA763682

• Gene Names: IL23A; P19; SGRF; IL-23; IL-23A; IL23P19
• Reactivity: Rat
• Synonyms: Interleukin-23 subunit alpha; Rat Interleukin-23 subunit alpha ELISA Kit; Il23a/IL-23/IL-23A/IL23P19/P19/SGRF/IL23A/SGRF/IL-23/IL-23A/IL-23-A/IL-23p19/interleukin 23 p19 subunit/interleukin 23; alpha subunit p19/interleukin-23 subunit alpha/Interleukin-23 subunit p19/JKA3 induced upon T-cell activation/SGRFIL-23 subunit alpha; Il23a elisa kit

• Reactivity: Rat
• Sequence Length: 189

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Il23a elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Il23a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Il23a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Il23a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Il23a can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7706702
• NCBI GeneID: 51561
• NCBI Accession #: NP_057668.1
• NCBI GenBank Nucleotide #: NM_016584.2
• Molecular Weight: 20,730 Da
• NCBI Official Full Name: interleukin-23 subunit alpha
• NCBI Official Synonym Full Names: interleukin 23 subunit alpha
• NCBI Official Symbol: IL23A
• NCBI Official Synonym Symbols: P19; SGRF; IL-23; IL-23A; IL23P19
• NCBI Protein Information: interleukin-23 subunit alpha
• UniProt Protein Name: Interleukin-23 subunit alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL23A
• UniProt Synonym Gene Names: SGRF; IL-23 subunit alpha; IL-23-A; IL-23p19
• UniProt Entry Name: IL23A_HUMAN

NCBI Description

This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. [provided by RefSeq, Jul 2008]

Uniprot Description

IL23A: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Belongs to the IL-6 superfamily.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 12q13.3

Molecular Function: cytokine activity; interleukin-23 receptor binding; protein binding

Biological Process: defense response to Gram-negative bacterium; defense response to virus; inflammatory response; innate immune response; negative regulation of interleukin-10 production; positive regulation of activated T cell proliferation; positive regulation of defense response to virus by host; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-17 production; positive regulation of memory T cell differentiation; positive regulation of natural killer cell activation; positive regulation of natural killer cell proliferation; positive regulation of NF-kappaB import into nucleus; positive regulation of NK T cell activation; positive regulation of NK T cell proliferation; positive regulation of osteoclast differentiation; positive regulation of T cell mediated cytotoxicity; positive regulation of T cell proliferation; positive regulation of T-helper 1 type immune response; positive regulation of tissue remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of tyrosine phosphorylation of Stat4 protein; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of tyrosine phosphorylation of Stat1 protein; T cell proliferation; tissue remodeling