Human S100 Calcium Binding Protein A13 ELISA Kit | S100A13 elisa kit

Human S100 Calcium Binding Protein A13 ELISA Kit

Cat. Num. AAA763626

• Reactivity: Human
• Synonyms: S100 Calcium Binding Protein A13; Human S100 Calcium Binding Protein A13 ELISA Kit; S100A13/S100 calcium binding protein A13/S100 calcium-binding protein A13protein S100-A13/S100 calcium-binding protein A13; S100A13 elisa kit

• Reactivity: Human
• Sequence Length: 98

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for S100A13 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human S100A13 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human S100A13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human S100A13 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human S100A13 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 47123484
• NCBI GeneID: 6284
• Molecular Weight: 11,471 Da
• NCBI Official Full Name: S100 calcium binding protein A13
• NCBI Official Synonym Full Names: S100 calcium binding protein A13
• NCBI Official Symbol: S100A13
• NCBI Protein Information: protein S100-A13
• UniProt Protein Name: Protein S100-A13
• Protein Family: Protein
• UniProt Gene Name: S100A13
• UniProt Entry Name: S10AD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A13: Plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; cytosol; extracellular space; mast cell granule; nucleus; perinuclear region of cytoplasm

Molecular Function: calcium ion binding; copper ion binding; fibroblast growth factor binding; lipid binding; protein binding; protein homodimerization activity; RAGE receptor binding; zinc ion binding

Biological Process: cytokine secretion; interleukin-1 alpha secretion; mast cell degranulation; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of cell shape; response to copper ion; response to electrical stimulus