Human Forkhead Box Protein C1 ELISA Kit | FOXC1 elisa kit

Human Forkhead Box Protein C1 ELISA Kit

Cat. Num. AAA763214

• Gene Names: FOXC1; ARA; IGDA; IHG1; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3
• Reactivity: Human
• Synonyms: Forkhead Box Protein C1; Human Forkhead Box Protein C1 ELISA Kit; FOXC1/ARA/FKHL7/FREAC3/IGDA/IHG1/IRID1/RIEG3/ARA/FKHL7RIEG3/forkhead box C1/forkhead box protein C1/forkhead; drosophila; homolog-like 7/forkhead/winged helix-like transcription factor 7/forkhead-related activator 3/Forkhead-related protein FKHL7/Forkhead-related transcription factor 3/FREAC-3/FREAC3IRID1/IGDA/IHG1/myeloid factor-delta; FOXC1 elisa kit

• Reactivity: Human
• Sequence Length: 553

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FOXC1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human FOXC1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human FOXC1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FOXC1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human FOXC1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119395716
• NCBI GeneID: 2296
• NCBI Accession #: NP_001444.2
• NCBI GenBank Nucleotide #: NM_001453.2
• Molecular Weight: 56,789 Da
• NCBI Official Full Name: forkhead box protein C1
• NCBI Official Synonym Full Names: forkhead box C1
• NCBI Official Symbol: FOXC1
• NCBI Official Synonym Symbols: ARA; IGDA; IHG1; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3
• NCBI Protein Information: forkhead box protein C1
• UniProt Protein Name: Forkhead box protein C1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXC1
• UniProt Synonym Gene Names: FKHL7; FREAC3; FREAC-3
• UniProt Entry Name: FOXC1_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXC1: Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Monomer. Expressed in all tissues and cell lines examined.

Protein type: Cell cycle regulation; Transcription factor; Cell development/differentiation; DNA-binding

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: cytoplasm; nuclear heterochromatin; nucleoplasm; nucleus

Molecular Function: DNA bending activity; DNA binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: artery morphogenesis; blood vessel remodeling; brain development; camera-type eye development; cardiac muscle cell proliferation; cell differentiation; cell migration; cell proliferation; collagen fibril organization; embryonic heart tube development; eye development; germ cell migration; glycosaminoglycan metabolic process; heart development; in utero embryonic development; lacrimal gland development; lymph vessel development; mesenchymal cell development; negative regulation of angiogenesis; negative regulation of mitotic cell cycle; neural crest cell development; Notch signaling pathway; odontogenesis of dentine-containing teeth; ossification; ovarian follicle development; paraxial mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of blood vessel size; regulation of organ growth; regulation of transcription, DNA-dependent; skeletal development; somitogenesis; transcription from RNA polymerase II promoter; ureteric bud development; vascular endothelial growth factor receptor signaling pathway; ventricular cardiac muscle morphogenesis

Disease: Axenfeld-rieger Syndrome, Type 3; Iridogoniodysgenesis, Type 1