Human Adenosine deaminase CECR1 ELISA Kit | CECR1 elisa kit

Human Adenosine deaminase CECR1 ELISA Kit

Cat. Num. AAA761620

• Gene Names: CECR1; PAN; ADA2; ADGF; IDGFL; SNEDS
• Reactivity: Human
• Synonyms: Adenosine deaminase CECR1; Human Adenosine deaminase CECR1 ELISA Kit; CECR1/Adenosine deaminase CECR1/Cat eye syndrome critical region protein 1/ADA2/ADGF/IDGFL; CECR1 elisa kit

• Reactivity: Human
• Sequence Length: 511

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CECR1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CECR1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CECR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CECR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CECR1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 532691757
• NCBI GeneID: 51816
• NCBI Accession #: NP_001269155.1
• NCBI GenBank Nucleotide #: NM_001282226.1
• Molecular Weight: 30,668 Da
• NCBI Official Full Name: adenosine deaminase CECR1 isoform a
• NCBI Official Synonym Full Names: cat eye syndrome chromosome region, candidate 1
• NCBI Official Symbol: CECR1
• NCBI Official Synonym Symbols: PAN; ADA2; ADGF; IDGFL; SNEDS
• NCBI Protein Information: adenosine deaminase CECR1
• UniProt Protein Name: Adenosine deaminase CECR1
• Protein Family: Adenosine deaminase
• UniProt Gene Name: CECR1
• UniProt Synonym Gene Names: ADA2; ADGF; IDGFL
• UniProt Entry Name: CECR1_HUMAN

NCBI Description

This gene encodes a member of a subfamily of the adenosine deaminase protein family. The encoded protein is one of two adenosine deaminases found in humans, which regulate levels of the signaling molecule, adenosine. The encoded protein is secreted from monocytes undergoing differentiation and may regulate cell proliferation and differentiation. This gene may be responsible for some of the phenotypic features associated with cat eye syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

CECR1: Adenosine deaminase that may contribute to the degradation of extracellular adenosine, a signaling molecule that controls a variety of cellular responses. Requires elevated adenosine levels for optimal enzyme activity. Binds to cell surfaces via proteoglycans and may play a role in the regulation of cell proliferation and differentiation, independently of its enzyme activity. Belongs to the adenosine and AMP deaminases family. ADGF subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Hydrolase; EC 3.5.4.4

Chromosomal Location of Human Ortholog: 22q11.2

Cellular Component: extracellular region; extracellular space

Molecular Function: adenosine deaminase activity; adenosine receptor binding; growth factor activity; heparin binding; protein homodimerization activity; proteoglycan binding; zinc ion binding

Biological Process: adenosine catabolic process; cellular protein metabolic process; hypoxanthine salvage; inosine biosynthetic process; multicellular organismal development

Disease: Polyarteritis Nodosa, Childhood-onset; Sneddon Syndrome