Goat anti-Human ZO-1 Polyclonal Antibody | anti-Tjp1 antibody

ZO-1 (Tight junction protein 1, Tight junction protein ZO-1, ZO1, ZO-1, Zona Occludens 1 Protein, Zonula Occludens 1 Protein)

Cat. Num. AAA623418

• Gene Names: Tjp1; ZO1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by peptide affinity chromatography.
• Synonyms: ZO-1; Polyclonal Antibody; ZO-1 (Tight junction protein 1; Tight junction protein ZO-1; ZO1; Zona Occludens 1 Protein; Zonula Occludens 1 Protein); Anti -ZO-1 (Tight junction protein 1; anti-Tjp1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZO-1. This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2).
• Purity/Purification: Affinity Purified; Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-Tjp1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Peptide ELISA Titer: 1:128,000; Western Blot: 0.3-1ug/ml ~170kD band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 187kD according to NP_783297.2).
• Immunogen: Synthetic peptide corresponding to C-PKPTSQNQFSEHDKT, from the internal region of human ZO-1 (NP_003248.3; NP_783297.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(MBS623418 (0.3µg/ml) staining of Human Cerebral Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-Tjp1 antibody

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.

Product Categories/Family for anti-Tjp1 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 303710
• NCBI GeneID: 21872
• UniProt Accession #: P39447; E9QK00; Q91YW2
• Molecular Weight: 194,742 Da
• NCBI Official Full Name: ZO-1
• NCBI Official Synonym Full Names: tight junction protein 1
• NCBI Official Symbol: Tjp1
• NCBI Official Synonym Symbols: ZO1
• NCBI Protein Information: tight junction protein ZO-1; OTTMUSP00000017707; zona occludens protein 1; zonula occludens protein 1
• UniProt Protein Name: Tight junction protein ZO-1
• Protein Family: Tight junction protein
• UniProt Gene Name: Tjp1
• UniProt Synonym Gene Names: Zo1
• UniProt Entry Name: ZO1_MOUSE

Uniprot Description

ZO1: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with tight junctions (TJ). May mediate dynamic changes in the composition of TJs. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting the protein kinase MRCKb to the leading edge of migrating cells. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Forms homodimers and heterodimers with ZO-2 or ZO-3. Interacts with occludin, CALM, claudins, CGN, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with MRCKb and MYZAP. Interacts (via PDZ domain) with Cx43. ZO-2 and ZO-3 shuttle between the TJ and the nucleus, where they may regulate gene expression. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins can be up- or downregulated in cancer and are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Alternative splicing produces two isoforms. The long isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Cellular Component: apical junction complex; intercellular canaliculus; cell surface; tight junction; apicolateral plasma membrane; basolateral plasma membrane; intercellular junction; adherens junction; cell-cell adherens junction; membrane; apical part of cell; apical plasma membrane; cytoplasm; gap junction; plasma membrane; cell junction; nucleus

Molecular Function: calmodulin binding; protein C-terminus binding; protein domain specific binding; protein binding

Biological Process: sensory perception of sound; blastocyst formation