Human Activating Transcription Factor 3 ELISA Kit | ATF-3 elisa kit

Human Activating Transcription Factor 3 ELISA Kit

Cat. Num. AAA761520

• Reactivity: Human
• Synonyms: Activating Transcription Factor 3; Human Activating Transcription Factor 3 ELISA Kit; ATF-3/JDP1/JUNDM1/SNFT/basic leucine zipper transcription factor; ATF-like 3/basic leucine zipper transcriptional factor ATF-like 3/B-ATF-3/FLJ36352/FLJ37535; 21 kDa small nuclear factor isolated from T-cells/JDP1/Jun dimerization protein 1/Jun dimerization protein p21SNFT/JUNDM1/SNFT21-kD small nuclear factor isolated from T cells; ATF-3 elisa kit

• Reactivity: Human
• Sequence Length: 181

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ATF-3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human ATF-3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ATF-3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ATF-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ATF-3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 442422
• NCBI GeneID: 467
• Molecular Weight: 14,314 Da
• NCBI Official Full Name: activating transcription factor 3
• NCBI Official Synonym Full Names: activating transcription factor 3
• NCBI Official Symbol: ATF3
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-3
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-3
• Protein Family: Activating transcription factor
• UniProt Gene Name: ATF3
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-3
• UniProt Entry Name: ATF3_HUMAN

NCBI Description

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]

Uniprot Description

ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleolus; nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor activity

Biological Process: cellular protein metabolic process; cellular response to amino acid starvation; gluconeogenesis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; unfolded protein response