Human Activating Transcription Factor 3 (ATF3) ELISA Kit | ATF3 elisa kit

Human Activating Transcription Factor 3 (ATF3) ELISA Kit

Cat. Num. AAA2025146

• Reactivity: Human
• Synonyms: Activating Transcription Factor 3 (ATF3); Human Activating Transcription Factor 3 (ATF3) ELISA Kit; Cyclic AMP-dependent Transcription Factor ATF3; ATF3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATF3. No significant cross-reactivity or interference between ATF3 and analogues was observed.
• Sequence Length: 181

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.060ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATF3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATF3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATF3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ATF3 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ATF3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ATF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ATF3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 442422
• NCBI GeneID: 467
• Molecular Weight: 14,314 Da
• NCBI Official Full Name: activating transcription factor 3
• NCBI Official Synonym Full Names: activating transcription factor 3
• NCBI Official Symbol: ATF3
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-3
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-3
• Protein Family: Activating transcription factor
• UniProt Gene Name: ATF3
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-3
• UniProt Entry Name: ATF3_HUMAN

NCBI Description

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]

Uniprot Description

ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; transcription corepressor activity; transcription factor activity

Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; transcription, DNA-dependent; unfolded protein response; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; gluconeogenesis