Human Intercellular adhesion molecule 3 ELISA Kit | ICAM-3 elisa kit

Human Intercellular adhesion molecule 3 ELISA Kit

Cat. Num. AAA761498

• Gene Names: ICAM3; CD50; CDW50; ICAM-R
• Reactivity: Human
• Synonyms: Intercellular adhesion molecule 3; Human Intercellular adhesion molecule 3 ELISA Kit; ICAM-3/CD50; ICAM-3 elisa kit

• Reactivity: Human
• Sequence Length: 547

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ICAM-3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ICAM-3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ICAM- 3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ICAM-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ICAM-3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 167466207
• NCBI GeneID: 3385
• NCBI Accession #: NP_002153.2
• NCBI GenBank Nucleotide #: NM_002162.3
• Molecular Weight: 59,541 Da
• NCBI Official Full Name: intercellular adhesion molecule 3
• NCBI Official Synonym Full Names: intercellular adhesion molecule 3
• NCBI Official Symbol: ICAM3
• NCBI Official Synonym Symbols: CD50; CDW50; ICAM-R
• NCBI Protein Information: intercellular adhesion molecule 3
• UniProt Protein Name: Intercellular adhesion molecule 3
• Protein Family: Intercellular adhesion molecule
• UniProt Gene Name: ICAM3
• UniProt Synonym Gene Names: ICAM-3
• UniProt Entry Name: ICAM3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008]

Uniprot Description

ICAM3: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. Belongs to the immunoglobulin superfamily. ICAM family.

Protein type: Membrane protein, integral; Immunoglobulin superfamily; Cell adhesion

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: integrin binding; protein binding; receptor binding

Biological Process: cell adhesion; cell-cell adhesion; extracellular matrix organization and biogenesis; innate immune response; regulation of immune response; stimulatory C-type lectin receptor signaling pathway