Human Ephrin-A1 ELISA Kit | EFNA1 elisa kit

Human Ephrin-A1 ELISA Kit

Cat. Num. AAA760973

• Gene Names: Efna1; B61; Efl1; Epl1; Eplg1; Lerk1; AI325262
• Reactivity: Human
• Synonyms: Ephrin-A1; Human Ephrin-A1 ELISA Kit; EFNA1/Ephrin A1/EFNA1/TNF alpha induced protein 4/LERK1/EFL1/B61/ECKLG/EPLG1/TNFAIP4/Tumor necrosis factor alpha-induced protein 4/TNF alpha-induced protein 4/EPH-related receptor tyrosine kinase ligand 1/LERK-1/Immediate early response protein B61; EFNA1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of EFNA1. No significant cross-reactivity or interference between EFNA1 and analogues was observed.
• Sequence Length: 205

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EFNA1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 247269690
• NCBI GeneID: 13636
• NCBI Accession #: NP_034237.3
• NCBI GenBank Nucleotide #: NM_010107.4
• Molecular Weight: 23,802 Da
• NCBI Official Full Name: ephrin-A1 isoform 1
• NCBI Official Synonym Full Names: ephrin A1
• NCBI Official Symbol: Efna1
• NCBI Official Synonym Symbols: B61; Efl1; Epl1; Eplg1; Lerk1; AI325262
• NCBI Protein Information: ephrin-A1
• UniProt Protein Name: Ephrin-A1
• Protein Family: Ephrin
• UniProt Gene Name: Efna1
• UniProt Synonym Gene Names: Epgl1; Epl1; Lerk1; LERK-1
• UniProt Entry Name: EFNA1_MOUSE

Uniprot Description

EFNA1: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis. Belongs to the ephrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Ligand, receptor tyrosine kinase; Cell development/differentiation; Tumor suppressor

Cellular Component: anchored to plasma membrane; extracellular region; membrane; plasma membrane

Molecular Function: ephrin receptor binding; protein binding

Biological Process: activation of MAPK activity; angiogenesis; axon guidance; cell migration; ephrin receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; notochord formation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of angiogenesis; regulation of axonogenesis; regulation of blood vessel endothelial cell migration; regulation of cell adhesion mediated by integrin; regulation of peptidyl-tyrosine phosphorylation