Human Ephrin A1 (EFNA1) ELISA Kit | EFNA1 elisa kit

Human Ephrin A1 (EFNA1) ELISA Kit

Cat. Num. AAA2024414

• Gene Names: Efna1; B61; Efl1; Epl1; Eplg1; Lerk1; AI325262
• Reactivity: Human
• Synonyms: Ephrin A1 (EFNA1); Human Ephrin A1 (EFNA1) ELISA Kit; B61; ECKLG; EFL1; EPLG1; LERK1; TNFAIP4; EPH-related receptor tyrosine kinase ligand 1; Tumor necrosis factor alpha-induced protein 4; Immediate early response protein B61; EFNA1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ephrin A1 (EFNA1).; No significant cross-reactivity or interference between Ephrin A1 (EFNA1) and analogues was observed.
• Sequence Length: 205

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin A1 (EFNA1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin A1 (EFNA1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EFNA1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin A1 (EFNA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin A1 (EFNA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin A1 (EFNA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ephrin A1 (EFNA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for EFNA1 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 247269690
• NCBI GeneID: 13636
• NCBI Accession #: NP_034237.3
• NCBI GenBank Nucleotide #: NM_010107.4
• UniProt Accession #: P52793; P97331
• Molecular Weight: 23,802 Da
• NCBI Official Full Name: ephrin-A1 isoform 1
• NCBI Official Synonym Full Names: ephrin A1
• NCBI Official Symbol: Efna1
• NCBI Official Synonym Symbols: B61; Efl1; Epl1; Eplg1; Lerk1; AI325262
• NCBI Protein Information: ephrin-A1
• UniProt Protein Name: Ephrin-A1
• Protein Family: Ephrin
• UniProt Gene Name: Efna1
• UniProt Synonym Gene Names: Epgl1; Epl1; Lerk1; LERK-1
• UniProt Entry Name: EFNA1_MOUSE

Uniprot Description

EFNA1: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis. Belongs to the ephrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Membrane protein, GPI anchor; Cell development/differentiation; Ligand, receptor tyrosine kinase

Cellular Component: anchored to plasma membrane; membrane; extracellular region; plasma membrane

Molecular Function: protein binding; ephrin receptor binding

Biological Process: axon guidance; cell migration; activation of MAPK activity; notochord formation; negative regulation of transcription from RNA polymerase II promoter; regulation of axonogenesis; regulation of blood vessel endothelial cell migration; regulation of angiogenesis; neuron differentiation; regulation of cell adhesion mediated by integrin; positive regulation of peptidyl-tyrosine phosphorylation; ephrin receptor signaling pathway; angiogenesis; regulation of peptidyl-tyrosine phosphorylation