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Typical Testing Data/Standard Curve (for reference only)

Human PIEZO1 (Piezo-type mechanosensitive ion channel component 1) ELISA Kit | PIEZO1 elisa kit

Human PIEZO1 (Piezo-type mechanosensitive ion channel component 1) ELISA Kit

Gene Names
PIEZO2; DA3; DA5; MWKS; DAIPT; FAM38B; HsT748; HsT771; FAM38B2; C18orf30; C18orf58
Reactivity
Human
Synonyms
PIEZO1 (Piezo-type mechanosensitive ion channel component 1); Human PIEZO1 (Piezo-type mechanosensitive ion channel component 1) ELISA Kit; FAM38A; KIAA0233; Mib; PIEZO1; PIEZO1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PIEZO1. No significant cross-reactivity or interference between PIEZO1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PIEZO1 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 233, 305 kDa
Observed: 235 kDa
NCBI Official Full Name
piezo-type mechanosensitive ion channel component 2
NCBI Official Synonym Full Names
piezo type mechanosensitive ion channel component 2
NCBI Official Symbol
PIEZO2
NCBI Official Synonym Symbols
DA3; DA5; MWKS; DAIPT; FAM38B; HsT748; HsT771; FAM38B2; C18orf30; C18orf58
NCBI Protein Information
piezo-type mechanosensitive ion channel component 2
UniProt Protein Name
Piezo-type mechanosensitive ion channel component 2
UniProt Gene Name
PIEZO2
UniProt Synonym Gene Names
C18orf30; C18orf58; FAM38B

NCBI Description

The protein encoded by this gene contains more than thirty transmembrane domains and likely functions as part of mechanically-activated (MA) cation channels. These channels serve to connect mechanical forces to biological signals. The encoded protein quickly adapts MA currents in somatosensory neurons. Defects in this gene are a cause of type 5 distal arthrogryposis. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]

Uniprot Description

FAM38B: Component of mechanosensitive channel required for rapidly adaptating mechanically activated (MA) currents in DRG neurons. Belongs to the PIEZO family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 18p11.22-p11.21

Cellular Component: plasma membrane

Molecular Function: cation channel activity; mechanically-gated ion channel activity

Biological Process: cation transport; detection of mechanical stimulus; detection of mechanical stimulus involved in sensory perception; regulation of membrane potential; response to mechanical stimulus

Research Articles on PIEZO1

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Product Notes

The Human PIEZO1 piezo2 (Catalog #AAA7608176) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7608176 ELISA Kit recognizes Human PIEZO1. It is sometimes possible for the material contained within the vial of "PIEZO1 (Piezo-type mechanosensitive ion channel component 1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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