Human TRIM5 (Tripartite motif-containing protein 5) ELISA Kit | TRIM5 elisa kit

Human TRIM5 (Tripartite motif-containing protein 5) ELISA Kit

Cat. Num. AAA7608148

• Gene Names: TRIM5; RNF88; TRIM5alpha
• Reactivity: Human
• Synonyms: TRIM5 (Tripartite motif-containing protein 5); Human TRIM5 (Tripartite motif-containing protein 5) ELISA Kit; Tripartite motif-containing protein 5 (EC:2.3.2.27); RING finger protein 88; RING-type E3 ubiquitin transferase TRIM5; TRIM5; RNF88; TRIM5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TRIM5. No significant cross-reactivity or interference between TRIM5 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRIM5 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 15011944
• NCBI GeneID: 85363
• NCBI Accession #: NP_149083
• NCBI GenBank Nucleotide #: NM_033092.1
• UniProt Accession #: Q9C035; Q2MLV4; Q2MLV8; Q2MLV9; Q2MLW1; Q2MLW3; Q2MLW4; A6NGQ1; A8WFA8; D3DQS8; D3DQS9; G3GJY1
• Molecular Weight: 29,677 Da
• NCBI Official Full Name: tripartite motif protein TRIM5 isoform gamma
• NCBI Official Synonym Full Names: tripartite motif containing 5
• NCBI Official Symbol: TRIM5
• NCBI Official Synonym Symbols: RNF88; TRIM5alpha
• NCBI Protein Information: tripartite motif-containing protein 5; ring finger protein 88; tripartite motif protein TRIM5; tripartite motif containing 5 transcript variant iota; tripartite motif containing 5 transcript variant kappa
• UniProt Protein Name: Tripartite motif-containing protein 5
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM5
• UniProt Synonym Gene Names: RNF88
• UniProt Entry Name: TRIM5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

TRIM5: Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. Belongs to the TRIM/RBCC family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; zinc ion binding; ubiquitin-protein ligase activity; pattern recognition receptor activity; ligase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; positive regulation of MAPKKK cascade; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of transcription factor activity; pattern recognition receptor signaling pathway; defense response to virus; activation of innate immune response; regulation of lipopolysaccharide-mediated signaling pathway; activation of NF-kappaB transcription factor