Human SFPQ (Splicing factor, proline- and glutamine-rich) ELISA Kit | SFPQ elisa kit

Human SFPQ (Splicing factor, proline- and glutamine-rich) ELISA Kit

Cat. Num. AAA7608134

• Gene Names: SFPQ; PSF; POMP100; PPP1R140
• Reactivity: Human
• Synonyms: SFPQ (Splicing factor; proline- and glutamine-rich); Human SFPQ (Splicing factor; proline- and glutamine-rich) ELISA Kit; 100 kDa DNA pairing protein; hPOMp100; POMP100; PSF; PTB associated splicing factor; SFPQ; SFPQ elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SFPQ. No significant cross-reactivity or interference between SFPQ and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SFPQ elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1709851
• NCBI GeneID: 6421
• UniProt Accession #: P23246; P30808; Q5SZ71
• Molecular Weight: Calculated MW: 72kDa/76kDa; Observed MW: 115kDa
• NCBI Official Full Name: Splicing factor, proline- and glutamine-rich
• NCBI Official Synonym Full Names: splicing factor proline/glutamine-rich
• NCBI Official Symbol: SFPQ
• NCBI Official Synonym Symbols: PSF; POMP100; PPP1R140
• NCBI Protein Information: splicing factor, proline- and glutamine-rich; hPOMp100; 100 kDa DNA-pairing protein; PTB-associated splicing factor; PTB-associated-splicing factor; DNA-binding p52/p100 complex, 100 kDa subunit; protein phosphatase 1, regulatory subunit 140; polypyrimidi
• UniProt Protein Name: Splicing factor, proline- and glutamine-rich
• Protein Family: Splicing factor
• UniProt Gene Name: SFPQ
• UniProt Synonym Gene Names: PSF; hPOMp100; PSF; PTB-associated-splicing factor
• UniProt Entry Name: SFPQ_HUMAN

Uniprot Description

PSF: a proline-and glutamine-rich splicing factor. Essential pre-mRNA splicing factor required early in spliceosome formation. Seems to also bind DNA. The active complex is a heterotetramer of two 52 kDa and two 100 kDa subunits. Reported to be a PKC-alpha-binding protein in the cell nucleus. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nucleus; paraspeckles; chromatin

Molecular Function: protein binding; histone deacetylase binding; nucleotide binding

Biological Process: alternative nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; RNA splicing; rhythmic process; negative regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; DNA repair; mRNA processing; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm; DNA recombination