Rabbit RNAse H2A Polyclonal Antibody | anti-RNAse H2A antibody

RNAse H2A Antibody

Cat. Num. AAA9414844

• Gene Names: RNASEH2A; AGS4; JUNB; RNHL; RNHIA; RNASEHI
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: RNAse H2A; Polyclonal Antibody; RNAse H2A Antibody; Rnse H2A; Ribonuclease H2 subunit A; RNAse HI large subunit; Aicadi-Goutieres; syndrome 4 protein; AGS4; anti-RNAse H2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 299

• Applicable Applications for anti-RNAse H2A antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 17 amino acid peptide near the center of human RNAse H2A.
• Target Name: RNAse H2A
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RNAse H2A in HeLa cell lysate with RNAse H2A antibody at 1 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of RNAse H2A in HeLa cells with RNAse H2A antibody at 2 ug/mL.)

Related Product Information for anti-RNAse H2A antibody

Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.

Product Categories/Family for anti-RNAse H2A antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 38455391
• NCBI GeneID: 10535
• NCBI Accession #: NP_006388.2
• NCBI GenBank Nucleotide #: NM_006397.2
• UniProt Accession #: O75792; Q96F11; B2RCY1
• Molecular Weight: 33,395 Da
• NCBI Official Full Name: ribonuclease H2 subunit A
• NCBI Official Synonym Full Names: ribonuclease H2, subunit A
• NCBI Official Symbol: RNASEH2A
• NCBI Official Synonym Symbols: AGS4; JUNB; RNHL; RNHIA; RNASEHI
• NCBI Protein Information: ribonuclease H2 subunit A
• UniProt Protein Name: Ribonuclease H2 subunit A
• UniProt Gene Name: RNASEH2A
• UniProt Synonym Gene Names: RNASEHI; RNHIA; RNase H2 subunit A; AGS4; RNase HI large subunit
• UniProt Entry Name: RNH2A_HUMAN

NCBI Description

The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.[provided by RefSeq, Aug 2009]

Uniprot Description

RNASEH2A: Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging- strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4). A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood. Belongs to the RNase HII family. Eukaryotic subfamily.

Protein type: EC 3.1.26.4; DNA replication; Ribonuclease

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleus

Molecular Function: RNA binding; metal ion binding; ribonuclease H activity; ribonuclease activity

Biological Process: mismatch repair; RNA catabolic process; DNA replication

Disease: Aicardi-goutieres Syndrome 4