Typical Testing Data/Standard Curve (for reference only)
Human GNPDA2 (Glucosamine-6-phosphate isomerase 2) ELISA Kit | GNPDA2 elisa kit
Human GNPDA2 (Glucosamine-6-phosphate isomerase 2) ELISA Kit
Gene Names
GNPDA2; GNP2; SB52
Reactivity
Human
Synonyms
GNPDA2 (Glucosamine-6-phosphate isomerase 2); Human GNPDA2 (Glucosamine-6-phosphate isomerase 2) ELISA Kit; GNPDA2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection ofGM-CSF. No significant cross-reactivity or interference betweenGM-CSF and analogues was observed.
Sequence Length
242
Samples
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/ml
Sensitivity
<9.375pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GMCSF were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GMCSF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GNPDA2 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GM-CSF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GM-CSF antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GM-CSF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofGM-CSF can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,956 Da
NCBI Official Full Name
glucosamine-6-phosphate isomerase 2 isoform 2
NCBI Official Synonym Full Names
glucosamine-6-phosphate deaminase 2
NCBI Official Symbol
GNPDA2
NCBI Official Synonym Symbols
GNP2; SB52
NCBI Protein Information
glucosamine-6-phosphate isomerase 2
UniProt Protein Name
Glucosamine-6-phosphate isomerase 2
UniProt Gene Name
GNPDA2
UniProt Synonym Gene Names
GNP2; GNPDA 2; GlcN6P deaminase 2
NCBI Description
The protein encoded by this gene is an allosteric enzyme that catalyzes the reversible reaction converting D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. Variations of this gene have been reported to be associated with influencing body mass index and susceptibility to obesity. A pseudogene of this gene is located on chromosome 9. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]
Research Articles on GNPDA2
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Product Notes
The Human GNPDA2 gnpda2 (Catalog #AAA7607241) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7607241 ELISA Kit recognizes Human GNPDA2. It is sometimes possible for the material contained within the vial of "GNPDA2 (Glucosamine-6-phosphate isomerase 2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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